FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254964
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG43120.3435955134968015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32910.26223859807930744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30060.23952878329577582No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA26640.21227700555553783No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25130.20024478789829828No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA25010.19928858517057063No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22950.18287377167791266No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22080.17594130190188723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21920.1746663649315837No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21770.17347111152192413No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACTCACTTTCGGCGGAGGGAC20620.1643075020478675No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19500.15538294325574278No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT18500.14741458719134573No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18440.1469364858274819No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18370.1463787009029741No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17180.1368963571863416No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16980.13530268597346218No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16950.1350636352915303No Hit
GTATGGTAGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT16760.13354964763929483No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16050.12789211483357293No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15360.12239394914913893No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT15060.12000344232981981No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14900.1187285053595163No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14810.11801135331372056No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14460.11522242869118157No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA14290.11386780816023408No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT13970.11131793421962702No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13950.11115856709833909No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA13790.10988363012803554No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAGTCTGGTACC13610.10844932603644407No Hit
GTATATTACTGTGCGAAAGGTGATTACGTTTGGGGGAGTTATCGTTATCC13560.10805090823322423No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13440.10709470550549656No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13170.10494324936810936No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13060.10406673020102569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5100.043.3838161
GGTATCA16650.041.5017471
TGGTATC5350.041.354892
CGTATGC2700.040.96024341
TCGTATG2800.039.42440
CACGAGA3200.038.30066721
ATCTCGT2400.037.2398337
GTATCAA38800.036.934731
ATGCCGT3250.036.32430344
TGCCGTC3200.035.85053345
GCCGTCT3200.034.80948646
GACTAGG3450.034.56599826
GAGACTA3650.034.51966524
CTAGGCA3500.034.11029428
CTTATAC3850.033.5975381
GTATGCC3650.033.18826342
GATCTCG2900.033.15251536
AGACTAG3750.032.70148525
TTATACA3900.032.29272
TAGGCAT3700.032.28583529
Produced by FastQC (version 0.11.5)