FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCATATATA61690.39154978565997306No Hit
ATTTATTACTGTGCGAGAGAGAAAGCAGGTCGGGCCTTCCTTCTAGACAA49700.3154486034576214No Hit
GTATTACTGTCAGCAATATGGTACCTCACGTCAACGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA43890.27857221741961774No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG35870.22766884116750258No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35460.2250665488653371No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34260.2174500835907064No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA34140.2166884370632433No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG33360.2117377346347334No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32630.20710438492599967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG32040.20335962283263959No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGTATAGGTAC31070.1972029800689798No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC28460.18063716809665803No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGC28370.1800659332010607No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27860.1768289354593427No Hit
GTACCTATACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAG27670.1756229951241928No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC27480.17441705478904296No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG26320.16705447169023327No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC25760.16350012122873897No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24630.15632794976179504No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24400.15486812725082416No Hit
CTCCAGAGACAACGCCAAGAACTCACTATATATGCAAATGAACAGCCTGAGAGACGAGGACACGGCTATTTATTA24210.1536621869156743No Hit
CTATATATGCAAATGAACAGCCTGAGAGACGAGGACACGGCTATTTATTA24150.15328136365194278No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24010.1523927760365692No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23900.15169460005306137No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG23780.1509329535255983No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23090.14655348599268567No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22820.14483978130589373No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22450.1424913711795493No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22380.14204707737186248No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT22200.1409046075806679No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC21770.1381753741905919No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21620.13722331603126303No Hit
GTGTATTACTGTCAGCAATATGGTACCTCACGTCAACGCAGTTTTGGCCA21190.13449408264118706No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21140.13417672992141078No Hit
GGGTTCCCTGGCCCCAGTTGTCTAGAAGGAAGGCCCGACCTGCTTTCTCTCTCGCACAGTAATAAATAGCCGTGT20590.1306858500038717No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT20540.13036849728409544No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC20450.12979726238849812No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC20190.1281470282456615No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG19510.12383103125670407No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC19330.12268856146550947No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG19220.12199038548200167No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG19210.1219269149380464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19190.12179997385013588No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18940.12021321025125449No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18710.11875338774028361No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT18610.11811868230073105No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18590.11799174121282054No Hit
GTCTAGAAGGAAGGCCCGACCTGCTTTCTCTCTCGCACAGTAATAAATAGCCGTGTCCTCGTCTCTCAGGCTGTT18550.11773785903699953No Hit
GGCCTGGAGTGGGTCTCATCCATTATTGGTAGTAGTATGTACATTGACTA18530.11761091794908901No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18530.11761091794908901No Hit
GCCTGAGAGACGAGGACACGGCTATTTATTACTGTGCGAGAGAGAAAGCAGGTCGGGCCTTCCTTCTAGACAA18450.11710315359744695No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAC18350.11646844815789441No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17830.1131679798722211No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17800.11297756824035533No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17470.1108830402898319No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17430.11062915811401086No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17360.11018486430632408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17320.10993098213050306No Hit
ATATGGTACCTCACGTCAACGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17160.10891545342721896No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG16980.10777298363602436No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGTCAATGT16840.10688439602065077No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGGCAGTGGCCCTGCT16760.10637663166900872No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16680.1058688673173667No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT16680.1058688673173667No Hit
GTATAGGTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCC16560.10510722078990362No Hit
GTACCATATTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC16530.10491680915803786No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG16530.10491680915803786No Hit
GAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGGTGGAGT16520.10485333861408258No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGCCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16440.10434557426244055No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16420.10421863317453003No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGAT16350.10377433936684323No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACC16200.1028222812075144No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16160.10256839903169337No Hit
GTACATTGACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAG16070.10199716413609608No Hit
GTCAATGTACATACTACTACCAATAATGGATGAGACCCACTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGGT15890.10085469434490148No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG15850.10060081216908044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15850.10060081216908044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.045.496191
GCTTGTA1150.038.6489371
TCGTATG2700.037.0276240
GTATCAA30950.036.564481
CTTATAC3000.036.468741
CGTATGC2850.036.32911341
GTAGTTT1401.0146978E-634.3158170
GGAGCTA3000.034.23726729
GTCGTAC501.963318E-434.189441
CGAGACG3050.033.63323223
ACGAGAC3050.033.63002822
CACGAGA3100.033.08445721
TATCAAC34500.032.90012
CCCACGA3300.032.1153219
ATGCCGT3050.031.78867744
ATCAACG35950.031.5731163
ATTTGCG652.4754687E-531.5494711
ATCTCCG3400.031.16283810
TAGTACG450.0048330230.389654
TACGGAT450.00483902630.3819357
Produced by FastQC (version 0.11.5)