FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCATATATA55520.35238846003958024No Hit
ATTTATTACTGTGCGAGAGAGAAAGCAGGTCGGGCCTTCCTTCTAGACAA46970.2981211449578365No Hit
GTATTACTGTCAGCAATATGGTACCTCACGTCAACGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA40850.25927717205722No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG32570.20672356166226816No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31940.20272491739308707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT31650.20088427161838465No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC30730.1950449815745011No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30200.19168104274487255No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28040.17797140525053728No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGC27940.1773366998109847No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27800.17644811219561116No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27350.17359193771762463No Hit
CTCCAGGCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGTATAGGTAC26580.16870470583306993No Hit
GTACCTATACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAG25890.16432523830015727No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC25590.1624211219814996No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25510.16191335762985756No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24510.155566303234332No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC24050.1526466582123902No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23250.14756901469596975No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22730.14426854641029643No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22610.14350689988283338No Hit
CTCCAGAGACAACGCCAAGAACTCACTATATATGCAAATGAACAGCCTGAGAGACGAGGACACGGCTATTTATTA21490.13639819895984473No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21390.13576349352029218No Hit
GGGTTCCCTGGCCCCAGTTGTCTAGAAGGAAGGCCCGACCTGCTTTCTCTCTCGCACAGTAATAAATAGCCGTGT21320.13531919971260536No Hit
CTATATATGCAAATGAACAGCCTGAGAGACGAGGACACGGCTATTTATTA21050.13360549502581348No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20900.13265343686648465No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20570.1305589089159612No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19690.12497350104789869No Hit
GTGTATTACTGTCAGCAATATGGTACCTCACGTCAACGCAGTTTTGGCCA19670.12484655995998817No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19530.12395797234461459No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19250.12218079711386744No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG18970.12040362188312026No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18690.1186264466523731No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18590.11799174121282054No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCAGCTACTTAGCCTGGTACC18580.1179282706688653No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAC18540.11767438849304426No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18520.11754744740513376No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG18320.11627803652602864No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT18120.11500862564692352No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18070.11469127292714723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17960.11399309694363942No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC17850.11329492096013162No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17830.1131679798722211No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG17780.11285062715244482No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG17620.11183509844916073No Hit
GTCTAGAAGGAAGGCCCGACCTGCTTTCTCTCTCGCACAGTAATAAATAGCCGTGTCCTCGTCTCTCAGGCTGTT17470.1108830402898319No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17460.11081956974587663No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17380.1103118053942346No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17360.11018486430632408No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17350.11012139376236883No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGAT17220.10929627669095049No Hit
GCCTGAGAGACGAGGACACGGCTATTTATTACTGTGCGAGAGAGAAAGCAGGTCGGGCCTTCCTTCTAGACAA17110.10859810070744268No Hit
GAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGGTGGAGT17020.10802686581184537No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGGCAGTGGCCCTGCT16780.10650357275691924No Hit
GTATAGGTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCC16700.10599580840527718No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCA16310.10352045719102222No Hit
GGCCTGGAGTGGGTCTCATCCATTATTGGTAGTAGTATGTACATTGACTA16110.1022510463119171No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16090.1021241052240066No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16050.10187022304818556No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT16010.10161634087236454No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGTCAATGT15840.10053734162512519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA400.00650181153.39460870
GGTATCA13850.040.971051
CGATTGT700.001155392640.68160670
GTATCAA32150.037.4266051
CGATTAA800.002226487335.59640570
TATCAAC36800.032.5962562
GTCACCG22250.031.99676770
ATCAACG38500.031.3165633
GGGTATC553.4383358E-431.0760691
CGCAGAG41700.028.5748588
GCTTGTA1202.9649527E-928.4863971
AGAGTAC41900.028.4375611
TCTGGAG27500.027.95935670
GCAGAGT42700.027.8256979
TTTAAGT2551.1305201E-725.12687370
GTGGTAT9750.024.8927291
TCCCGTA1751.4203425E-424.40896270
GAGTACT10650.024.36611412
AATCGTT851.4614803E-424.30885739
TGGTATC9900.024.1641332
Produced by FastQC (version 0.11.5)