FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531064
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGG101300.6616313883678279No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC81990.535509946024464No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG81450.5319829869946651No Hit
GCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCCA53020.3462951254813646No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48260.3152056347742485No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46650.30469007174095925No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42600.2782378790174676No Hit
GTCCTGGGCCCAGCCTGTACTGACTCAGCCACACTCAGCGTCGGGGACCC40030.2614521665978692No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39260.2564229842776004No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT38680.252634769023372No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35760.23356306464001503No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34690.22657446063652467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34510.2253988076265917No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTC32720.2137075915833695No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32550.2125972526295439No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30340.19816284622981142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29630.19352554824618695No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29370.19182738278739492No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29250.19104361411410628No Hit
GTCTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATTAACAGAATTCGTT28870.18856167998202558No Hit
GTGCAGGGTCCTGGGCCCAGCCTGTACTGACTCAGCCACACTCAGCGTCG28870.18856167998202558No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28850.1884310518698108No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28690.1873860269720926No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28180.1840550101106159No Hit
AGCCTGTACTGACTCAGCCACACTCAGCGTCGGGGACCCCCGGGCAGAGG27370.17876457156591757No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27110.17706640610712548No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27090.17693577799491073No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC26900.17569481092887038No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26560.17347413302121922No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATTAA26410.17249442217960842No Hit
GTACTGACTCAGCCACACTCAGCGTCGGGGACCCCCGGGCAGAGGGTCAC25590.16713866957880272No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25410.16596301656886975No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG24820.16210948725853394No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24450.15969286718256062No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23750.15512088325504356No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23490.1534227177962515No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22330.14584628728779464No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22270.1454544029511503No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22240.14525846078282814No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTACTGACTCAGCCAC21960.14342966721182132No Hit
CCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCCGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21360.1395108238453781No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20580.13441632746900195No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20390.1331753604029616No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCCAGAGAATCGGGCAGGGACC20380.13311004634685422No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20080.13115062466363261No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCGG19580.12788492185826328No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19430.12690521101665247No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19240.12566424395061213No Hit
GGATGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTGGGTCAGCCCAAGGCTGCCCC18890.1233782519868536No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTCCGCCGAACAC18880.12331293793074621No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18840.12305168170631665No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18470.12063506163034333No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17430.11384239979517512No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCCAGAGAATCGGGC17420.11377708573906772No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGC17260.11273206084134954No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17240.11260143272913477No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17220.11247080461692001No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17160.11207892028027569No Hit
TTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGGCG17050.11136046566309443No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGCTTGGTC16690.10900915964322849No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16530.10796413474551032No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16500.10776819257718814No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16490.10770287852108076No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16200.10580877089396655No Hit
ATGTTGGACCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT16070.10495968816457053No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16020.1046331178840336No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC15990.10443717571571143No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15960.10424123354738928No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15580.10175929941530856No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA15580.10175929941530856No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15440.10084490262980517No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15360.10032239018094606No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15360.10032239018094606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.050.765661
TGGTATC4550.047.97322
GGTATCA17250.046.0707551
TATACAC4200.042.226413
TCGTATG2550.041.9444140
TTATACA4150.041.09152
GTATCAA44250.038.9257161
CTTATAC4450.038.3237951
CGTATGC3000.037.99687241
TCTCGTA2300.037.4077138
CACGAGA4400.036.42421321
GGATGGG6350.035.3609270
CCCTAAG4700.034.8783228
ATGCCGT3200.034.6607244
TAAGACA4550.034.58240531
TATCAAC49650.034.208992
AGGGGGG3800.034.0214970
ATCTCGT2700.033.1033637
TGCCGTC3150.033.0420245
ATCAACG52950.032.2058073
Produced by FastQC (version 0.11.5)