FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531064
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGG95610.6244676904427248No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC76380.49886876054822005No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47650.3112214773516979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45600.2978320958496836No Hit
GTCCTGGGCCCAGCCTGTACTGACTCAGCCACACTCAGCGTCGGGGACCC44140.28829624365800516No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43420.2835936316182733No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG40840.2667426051425675No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39450.25766395134364073No Hit
GCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTCCGCCGAACACCCA38010.24825872726417708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36200.23643688310874006No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34590.2259213200754508No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34280.2238965843361218No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33410.21821426145477918No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32140.20991937632914107No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32030.2092009217119598No Hit
AGCCTGTACTGACTCAGCCACACTCAGCGTCGGGGACCCCCGGGCAGAGG31950.2086784092631007No Hit
GTGCAGGGTCCTGGGCCCAGCCTGTACTGACTCAGCCACACTCAGCGTCG31540.20600053296269782No Hit
GTCTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATTAACAGAATTCGTT31050.2028001442134359No Hit
GTACTGACTCAGCCACACTCAGCGTCGGGGACCCCCGGGCAGAGGGTCAC28870.18856167998202558No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28450.18581848962551534No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27830.18176901814685736No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27160.17739297638766244No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGAC27070.17680514988269597No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTC26740.1746497860311522No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26530.17327819085289706No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25900.1691634053181317No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24990.1632198262123595No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG24740.16158697480967485No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24590.16060726396806405No Hit
CCCCTGAGGGCCGCTGATAATTATTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAATTAA24480.1598888093508828No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24280.15858252822873506No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24000.1567537346577282No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGCCTGTACTGACTCAGCCAC23900.15610059409665436No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22890.1495038744298083No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22620.14774039491490884No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22530.14715256840994237No Hit
CCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCCGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT22440.1465647419049759No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22390.14623817162443895No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22250.14532377483893552No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21650.14140493147249233No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA21630.14127430336027758No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGCCAGCTTGGTCCCTCCGCCGAACAC20260.13232627767356558No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20190.13186907928081384No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19980.13049748410255874No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19950.13030154193423657No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19200.12540298772618258No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGAAACGAATTCTGTTAATTGGTACCAGCAACTCCCAGGAACGGCCCC18980.12396607849182006No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18310.11959003673262517No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCGG18260.11926346645208823No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17810.11632433392725582No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17660.11534462308564501No Hit
GGATGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTGGGTCAGCCCAAGGCTGCCCC17450.1139730279073899No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGC17310.11305863112188648No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC17290.1129280030096717No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA17250.11266674678524216No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC17120.11181766405584613No Hit
TTATTACTGTGCGGCATGGGATGACAGCCTGAATGGCTGGGTGTTCGGCG16830.10992355642873192No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16560.10816007691383248No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16560.10816007691383248No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCGGACTTGGAGCCAGAGAATCGGGC16360.10685379579168475No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16340.10672316767946996No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC16330.10665785362336258No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15930.10404529137906711No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15890.10378403515463755No Hit
GCATGGGATGACAGCCTGAATGGCTGGGTGTTCGGCGGAGGGACCAAGCTGGCCGTCCTGGGTCAGCCCAAGG15800.10319620864967108No Hit
ATGTTGGACCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT15550.1015633572469864No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15510.10130210102255686No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15450.10091021668591255No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15340.10019176206873129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5050.047.8981931
GGTATCA17700.044.0772321
TGGTATC5550.042.969192
GTATCAA47050.039.535311
TATCAAC52800.035.0362852
ATCAACG56300.032.8431553
TCAACGC56350.032.750374
AACGCAG57250.032.530776
CAACGCA57500.032.153535
ATAAACG652.5385667E-531.4333973
ACGCAGA61050.030.5059227
CGCAGAG61300.030.3259648
AGAGTAC61900.030.02711111
CAGAGTA62450.029.81814610
GCAGAGT63500.029.3825389
GTACGGT500.00824144927.24405914
GGATGGG7300.026.0168670
TAGGCAA4650.025.71638370
CATGCCG23450.025.49705370
GAGTACG53800.024.87178412
Produced by FastQC (version 0.11.5)