FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839391
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTC29440.35073047006698904No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26370.3141563347712806No Hit
GGCTAACACTTTCCCGTGGACGTTCGGCCAAGGGACCAACGTGGAAATCAGACGAACTGTGGCTGCACCAT21990.26197564662952066No Hit
GGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGATACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCATC21500.25613808106115027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18360.21873000782710322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17990.2143220501530276No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17450.20788881462870104No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17090.20359999094581668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16780.19990683721888844No Hit
GTCTTCAGGCTGCAGGCTGCTGATGTTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC16100.19180572581788463No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15310.18239414051377725No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15200.18108366661067368No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGATA14210.16928940148274166No Hit
GTTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG13990.16666845367653454No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13710.16333270192318No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACGTTGGTCCCTTGGCCGAACGTC12780.15225324074239538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12780.15225324074239538No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12490.14879835499784963No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12270.14617740719164252No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCGTCAAGGTT12020.1431990574118617No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAAATACA11970.14260338745590553No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11840.1410546455704195No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT11840.1410546455704195No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11770.1402207076320809No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11680.13914850171135978No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11580.13795716179944745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11490.13688495587872634No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10870.12949864842486994No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10830.129022112460105No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10710.1275925045658102No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10250.12211234097101352No Hit
GCCTGAAGACTTTGCAACTTACTATTGTCAACAGGCTAACACTTTCCCGT10220.1217549389974398No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9830.11710871334098175No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9770.11639390939383433No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATTTCCACGTTGGTCC9750.11615564141145188No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTATCTGCTGATACCAGGC9490.11305815764047983No Hit
ATATATTACTGTGTGAGAGATCGCAGAGTACAACTTTCGTACTACTTTGA9450.1125816216757149No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT9370.11162854974618504No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9110.10853106597521298No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9050.1078162620280656No Hit
GCCACAGTTCGTCTGATTTCCACGTTGGTCCCTTGGCCGAACGTCCACGG8900.10602925216019711No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8830.10519531422185846No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8800.10483791224828477No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8800.10483791224828477No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTATCTGCTGATACCAGGCTAACC8650.10305090238041628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)