FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37410.26059008573490444No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33190.23119446526440735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29230.20360994937266125No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27060.1884941919269317No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25320.17637372282298264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25300.17623440708615562No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22300.15533704656210556No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC22240.15491909935162457No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21860.15227210035191155No Hit
CTATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT21290.14830160185234204No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21200.14767468103662051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20850.14523665564214805No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20370.1418930779583No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19860.1383405266692115No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCACTATAGTAACTGG18920.13179268703834246No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18720.13039952967007248No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18520.12900637230180248No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT18470.12865808295973497No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT18300.12747389919670546No Hit
ATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGA17620.12273716414458744No Hit
GTTACTATAGTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT17280.12036879661852842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17270.12029913875011493No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17050.11876666564501794No Hit
CTATAGTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCT16780.11688590319785341No Hit
GCACTATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT16760.11674658746102644No Hit
GTTTATTACTGTCAGCACTATAGTAACTGGCCCACCTTCGGCCAGGGGAC16530.11514445648751592No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16250.11319403617193792No Hit
CTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT16230.11305472043511092No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCA15930.11096498438270591No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15840.11033806356698439No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15470.10776072243568488No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT15110.10525303917279889No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15030.10469577622549088No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15020.10462611835707737No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14630.10190946148895087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7450.047.485481
CTACGCT3750.039.6730228
GTATCAA57800.038.821671
GGTATCA24000.038.184361
TGCATCG1900.037.64650334
TACGCTG4000.037.21687729
CACGAGA4400.035.239521
CTTATAC4500.034.4407961
GTGCGAA400.002919905133.6838659
ATCTCCG4650.033.32167410
GACAGGT4100.032.8703231
CTCTTAT1850.032.7814871
TCTCCGA4650.032.5972911
TATACAC5050.032.0219233
TATCAAC71050.032.006432
CCCACGA4850.031.95983119
TACACAT5200.031.7460965
ATCAACG71750.031.6941763
AACGCAG72400.031.5026956
TCAACGC73100.031.1088524
Produced by FastQC (version 0.11.5)