FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49510.34487610651523976No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32820.22861712413310784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29060.2024257656096317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27160.18919077061106668No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25500.17762756445442565No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23310.1623724912718691No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC20630.14370418253705103No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19850.138270868800798No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19060.13276789719613147No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18650.12991192459117798No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA18520.12900637230180248No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18490.12879739869656195No Hit
CTATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT18300.12747389919670546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18120.12622005756526244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18080.12594142609160844No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17810.12406066364444394No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT17520.12204058546045245No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTCTGCAGTTTATTACTGTCAGCACTATAGTAACTGG17490.12183161185521194No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17010.11848803417136393No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16540.11521411435592942No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16370.11402993059289991No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT15910.11082566864587891No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15880.1106166950406384No Hit
ATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGA15210.10594961785693388No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14940.10406885540976939No Hit
GTCCCAGACCCACTGCCACTAAACCTGGCTGGGATACCAGTGGCCCTGGT14730.10260604017308587No Hit
GTTACTATAGTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT14700.10239706656784536No Hit
CTGGTATCCCAGCCAGGTTTAGTGGCAGTGGGTCTGGGACAGAGTTCACT14620.10183980362053738No Hit
GCACTATAGTAACTGGCCCACCTTCGGCCAGGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT14610.10177014575212387No Hit
CTATAGTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCTGCT14390.10023767264702686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6450.043.8865931
GTATCAA53650.040.8277971
GGTATCA20600.038.4426161
GACCGGA1951.5643309E-1037.47822670
TATCAAC66550.033.098992
ATCAACG66300.033.005643
TCAACGC67550.032.3824584
AACGCAG67850.032.3362246
CAACGCA68050.032.142295
GATTCAC2100.032.0939371
ACGCAGA70650.031.0546767
CGCAGAG71100.030.9054558
AGAGTAC73150.029.85325411
GCAGAGT74050.029.719699
TAAACCG705.635782E-428.56093268
CAGAGTA78400.027.8970710
AGACCGG1907.075869E-1026.15959769
GAGTACG68750.025.65137712
AGTACGG68950.025.48201213
GACAGGT3600.025.2739731
Produced by FastQC (version 0.11.5)