FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193495
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCACATACA42480.3559294341409055No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37080.31068416708909546No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29960.25102744460596815No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29650.248430031127068No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29220.24482716726923867No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27740.2324266125957796No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCACATA23990.20100628825424488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23460.1965655490806413No Hit
ATATTACTGTGCGAAAGGTCGATATATCAGCAGCTATTACTTGGATGACTCCTGGGGCCAGGGAACCCTGGTCAC22910.19195723484388288No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22380.1875164956702793No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21980.1841649944071823No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT20380.1707589893547941No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC20210.16933460131797787No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20070.16816157587589392No Hit
CTATATTACTGTGCGAAAGGTCGATATATCAGCAGCTATTACTTGGATGA19540.16372083670229035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19260.1613747858181224No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATT19190.1607882730970804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18570.15559344613928003No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18290.15324739525511208No Hit
GCCCTATATTACTGTGCGAAAGGTCGATATATCAGCAGCTATTACTTGGA18090.15157164462356357No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17850.14956074386570536No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17270.14470106703421465No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16580.13891972735537225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16460.13791427697644312No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT16410.137495339318556No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16390.13732776425540116No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT16200.13573580115543007No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16040.13439520065019125No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15940.133557325334417No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCT15700.13154642457655877No Hit
GTGTTAGCACCTACTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCC15680.1313788495134039No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCAATTGCACATACAGCG15380.12886522356608113No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15110.12660296021349063No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15010.12576508489771637No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCATCCAAGTAATAGCTGCTGATATATCGACCTTT14930.12509478464509696No Hit
GTATTAGTGGTGGTGGTGGTAGCACAGACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT14790.12392175920301299No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT14760.12367039660828073No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13590.11386725541372188No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13580.11378346788214445No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13470.11286180503479278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13390.11219150478217335No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTT13200.11059954168220226No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT13190.11051575415062485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12900.1080859157348795No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12800.10724804041910524No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC12760.10691289029279552No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC12730.10666152769806325No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12450.10431547681389532No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12220.10238836358761454No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCG12150.10180185086657255No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC12110.10146670074026283No Hit
CCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGAC12070.10113155061395314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1750.045.28159340
GGTATCA9250.043.37111
CTAGATC1500.043.2861433
ACCGTAC1850.042.31100527
CGTACTA1900.041.23734729
GACCGTA1900.041.19064726
CGTATGC1950.040.69609541
TACTAGA1850.040.5735331
ACTAGAT1900.039.53906632
GTACTAG2000.039.20841230
GATCTCG1600.038.55862836
ACGAGAC2150.037.9518522
ATGCCGT2050.037.1748944
CTTATAC2150.036.370521
ATCTCGT1700.036.3426837
GTATGCC2200.036.1068642
ATAGGCT400.002345445635.2223153
TAGATCT1850.035.13238534
GTATCAA21450.034.870291
TGTTAGA7050.034.37401270
Produced by FastQC (version 0.11.5)