FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT98080.6459188505173343No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTC71710.47225571748162776No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT55110.36293421545687493No Hit
CTTTCATACAGCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAA54420.35839012892693045No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54150.3566120081108652No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTGTATGAAAGACT51890.34172847831713377No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50190.3305329028085748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38560.2539419950647269No Hit
GTATGAAAGACTCTGACTGGACTTGCAGCTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA37010.24373426445398189No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36090.23767548241405584No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35460.23352653384323693No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32000.21074024486699328No Hit
CTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTT31700.2087645550713652No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGA30990.20408875588837883No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30860.2032326236436066No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATA30640.20178378446014605No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29680.19546157711413625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28840.18992964568637768No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28400.18703196731945654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28270.18617583507468438No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27940.1840025762994935No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27920.18387086364645164No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27550.18143417956517702No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25780.16977760977097145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25140.1655628048736316No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25010.16470667262885944No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24620.16213827589454294No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATACAGCTCCAA24530.16154556895585453No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24370.16049186773151955No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA24130.1589113158950171No Hit
GTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23600.15542093058940754No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA23070.15193054528379796No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23050.15179883263075608No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22350.1471888897742906No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA22330.14705717712124874No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC22180.1460693322234347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21750.1432375101830345No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCT21210.139681268550904No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTT20610.13572988895964785No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20140.13263464161316388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20010.13177850936839172No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19960.13144922773578707No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19890.13098823345014052No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19020.12525873304281912No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTG19010.1251928767162982No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATACAGCTCCAACAGCAAGACCTA18870.12427088814500509No Hit
ATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA18870.12427088814500509No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18740.12341475590023293No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGT18360.12091221549243739No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18310.12058293385983272No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTG18310.12058293385983272No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18150.11952923263549775No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17960.11827796243159996No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17410.11465586447294852No Hit
GCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG17160.11300945630992514No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16820.11077034120821334No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16790.11057277222865053No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16740.11024349059604585No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16610.10938735835127371No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16130.1062262546782688No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16000.10537012243349664No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15970.10517255345393382No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT15960.10510669712741288No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15880.1045798465152454No Hit
GACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC15730.10359200161743137No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC15710.10346028896438951No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15580.10260415671961734No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15300.10076017957703115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2450.046.47381641
ATCGTAT306.4183044E-445.8444539
ATCTCGT2500.043.87574837
GCGAATC3750.043.67344733
GTGGTAT3050.043.3226051
CGAATCT3000.043.2558234
TCGTATG2400.043.0525740
ATGCCGT2850.042.58242844
TATGCCG2800.042.03644643
ACTAAGG5100.041.9283427
GTATGCC2900.041.71606442
GGTATCA11000.041.5806161
GGCGAAT4600.041.49732632
TAAGGCG5100.041.32417329
CCGTCTT3050.041.1363947
GACTAAG5250.040.71292526
TGCCGTC3150.039.69731545
CTTATAC5300.039.6337931
AGGCGAA4900.039.60638831
GCCGTCT2900.039.58502246
Produced by FastQC (version 0.11.5)