FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT89090.5867140129750135No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTC78850.5192771346175756No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54540.35918040484518166No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51700.340477208113236No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTGTATGAAAGACT45700.3009634122006748No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT45580.30017313628242354No Hit
CTTTCATACAGCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAA39720.2615813289411554No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGA37480.24682951180046586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36770.24215371261747948No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36650.24136343669922825No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36420.2398487411892467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34130.22476764241595254No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30560.2012569338479786No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30130.19842511180757832No Hit
CTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTT29580.1948030138489269No Hit
GTATGAAAGACTCTGACTGGACTTGCAGCTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29330.19315660568590354No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCG26600.17517782854568814No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26500.1745192652804788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26110.17195086854616232No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25930.17076545466878548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25620.16872390854663646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25600.16859219589359462No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATA24270.15983330446631022No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24200.15937231018066367No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23800.15673805711982625No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCGGATTCCCGGGT23690.15601363752809594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23610.15548678691592846No Hit
GTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23540.1550257926302819No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23150.15245739589596546No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22860.15054756242685832No Hit
GCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGT22740.1497572865086071No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22700.14949386120252336No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22480.14804502201906278No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22210.1462669012029975No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21610.14231552161174138No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA21350.14060325712219707No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA21190.1395495558978621No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC21100.13895684895917368No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21020.1384299983470062No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20900.13763972242875497No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATACAGCTCCAA20790.1369153028370247No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCT20480.1348737567148757No Hit
TCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACC20320.13382005549054074No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA19100.1257855836549866No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18640.12275619263502358No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTG18290.12045122120679085No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18160.11959508896201869No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18120.11933166365593494No Hit
GAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAAT17770.1170266922277022No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17630.11610470365640911No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17540.11551199671772068No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTT17510.11531442773815789No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17430.1147875771259904No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16870.11109962284081801No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16860.11103376651429707No Hit
ATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA16620.10945321467779462No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16310.10741166855564564No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16190.1066213926373944No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16160.10642382365783161No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGT16070.10583111671914319No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15760.10378957059699419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3250.046.8801571
TGGTATC3600.042.300042
GGTATCA11250.040.629471
GTATCAA28250.038.8317031
GACAGGT2150.034.6452831
TATCAAC33200.032.61692
ATCAACG33450.032.3581963
CGATGTG450.003734039432.03454261
TGTTAGA17250.031.90718870
TCAACGC34000.031.823224
CAACGCA34850.031.0460225
AACGCAG35450.030.7113136
CTATACC450.005101658430.0552644
ACGCAGA36750.029.5329277
TCCGAGA8600.029.4736370
CGCAGAG37200.028.993898
AGAGTAC38000.028.4687211
ACTACGG500.007149359728.0492950
GCAGAGT38850.027.9365529
CAGAGTA39150.027.54884510
Produced by FastQC (version 0.11.5)