FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965751
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31510.32627457802269944No Hit
GTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGA29120.3015269981599812No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21830.22604170226072764No Hit
GTCTAGGACAGACTTCACTCTCACGATCAGCAGACTGGAGCCTGAAGATT21420.22179630153114No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20270.20988847021644297No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20160.2087494602646024No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCTAGACCCACTGCCA19710.20408987409798177No Hit
GTCCTAGACCCACTGCCACTGAGCCTGTCTGGGATGCCAGTGGCCCTCCT18630.19290686729809237No Hit
GTGTATTACTGTGCGAAAGATCAGGTTGTTGCGGTCTCGGGCCTTGATGC17540.18162031413894472No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16290.1686770192316653No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15330.15873656874287473No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15150.15687273427622647No Hit
CTCCTGTGCAGCCTCTGGATTGACCTTCAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14420.1493138500503753No Hit
GTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14370.14879611825408412No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14320.14827838645779295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.1480712937392765No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14200.1470358301466941No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14140.14641455199114473No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA13700.14185851218378237No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13470.13947694592084295No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13240.13709537965790355No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13160.13626700878383766No Hit
CATCTATGGTGCATCGAGGAGGGCCACTGGCATCCCAGACAGGCTCAGTGGCAGTGGGTCTAGGACAGACTTCA12960.13419608159867294No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCTAGACCCACTGCCA12090.12518754834320647No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12050.12477336290617354No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC11900.1232201675173No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11890.12311662115804178No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11520.11928540586548705No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11130.11524709785441589No Hit
GTATTACTGTGCGAAAGATCAGGTTGTTGCGGTCTCGGGCCTTGATGCTT10860.11245134615444355No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10730.11110524348408649No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTGACCTTCAGTAGCTATGCCATGAGCTGGGTCCG10640.11017332625076236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10540.10913786265818001No Hit
CCATTGTCCCTTGGCCCCAGAGATCAAAAGCATCAAGGCCCGAGACCGCA10530.10903431629892177No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10380.10748112091004823No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10370.10737757455079001No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10370.10737757455079001No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10330.10696338911375707No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10190.10551374008414176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10170.1053066473656253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10140.10499600828785059No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10110.10468536921007587No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT10070.10427118377304295No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGAGATCAAAAGCATCAAGGC9990.10344281289897707No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTC9880.10230380294713648No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9870.10220025658787824No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9850.10199316386936177No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9800.1014754320730706No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9760.10106124663603765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATT400.00748688351.52045470
TCGTATG2400.043.1604240
TATACAC4350.042.4543573
TCTGGAG13250.040.43869470
CTTATAC4500.040.2792241
GCCGTCT2900.039.48906346
TGAGCGA4350.039.4341531
GTCGTAT1050.039.4197439
GAGCGAT3850.039.22919532
ACGAGAC4450.039.1967822
ATCTCGT1950.038.83525537
CGAGACA4500.038.76326823
CGTATGC2950.038.66534841
TTATACA4800.038.474262
GTATCAA25350.038.4489671
GGTATCA9700.038.430191
ATCTCCG4650.038.23650410
CCGTCTT3300.037.91306747
CGTATTA654.9416485E-737.62101752
ATGCCGT3100.036.8793744
Produced by FastQC (version 0.11.5)