FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965751
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34540.35764912487794476No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA28930.2995596173340747No Hit
GTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGA26820.2777113355305871No Hit
GTCTAGGACAGACTTCACTCTCACGATCAGCAGACTGGAGCCTGAAGATT19950.20657498672017943No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19250.19932674157210295No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18870.19539197992029No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18860.1952884335610318No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCTAGACCCACTGCCA18700.19363169181290002No Hit
GTGTATTACTGTGCGAAAGATCAGGTTGTTGCGGTCTCGGGCCTTGATGC18100.1874189102574059No Hit
GTCCTAGACCCACTGCCACTGAGCCTGTCTGGGATGCCAGTGGCCCTCCT16280.16857347287240707No Hit
CTCCTGTGCAGCCTCTGGATTGACCTTCAGTAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14410.14921030369111707No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13080.1354386379097718No Hit
GTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12840.13295352528757415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12730.13181451533573355No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA12420.12860457819872825No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12310.12746556824688765No Hit
CATCTATGGTGCATCGAGGAGGGCCACTGGCATCCCAGACAGGCTCAGTGGCAGTGGGTCTAGGACAGACTTCA12190.12622301193578883No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12170.12601591921727234No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12140.12570528013949764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11990.12415208475062413No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11970.12394499203210767No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11760.1217705184876847No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCTAGACCCACTGCCA11720.12135633305065177No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA11690.12104569397287707No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10930.11317617066925117No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10870.11255489251370177No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10440.10810239906559765No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC10430.10799885270633942No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGT10390.10758466726930647No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10240.10603147188043295No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9970.10323572018046061No Hit
GTATTACTGTGCGAAAGATCAGGTTGTTGCGGTCTCGGGCCTTGATGCTT9880.10230380294713648No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9790.10137188571381235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.038.6570051
TCTGGAG12700.038.56053570
GTATCAA21750.038.4864541
AACTGTG14400.034.0082570
TATCAAC25100.033.3429032
ATCAACG25250.032.8606383
TCAACGC25600.032.2713054
CAACGCA25950.031.9659485
AACGCAG26350.031.8693456
CGAGCGA553.1652796E-431.51614245
TGTACGA450.00456405430.74626742
CGCAGAG28250.029.8467568
AGAGTAC28350.029.50065611
GACAGGT3150.029.285611
CGACCAA605.138175E-429.0148949
GCAGAGT28950.029.0071589
GAGCGAC605.24227E-428.9156446
GGCGATC500.00659890428.5122664
GGGACAA8950.027.7550970
CGGCTCT10750.026.70173321
Produced by FastQC (version 0.11.5)