FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137102
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT76510.35800818117244754No Hit
GATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGG70040.3277335382213858No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC62960.29460456262733364No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC62540.2926392844141272No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCA60700.2840294941467464No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG60700.2840294941467464No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG57550.26928990754769777No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA56540.2645638813683203No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC53040.2481865629249329No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC51220.2396703573343715No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC48820.22844019611604874No Hit
GATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGG46620.21814588166591953No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT44270.20714968213964516No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC43040.2013942245152548No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42640.19952253097886763No Hit
CTATGTGGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAACGGCAAAGACTCACTGTATCTGCAAATGAA41590.19460933544585143No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT40850.19114670240353526No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG39610.18534445244073516No Hit
GTCCTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG39430.18450219034936097No Hit
GCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCACTTCCGAAGACGTACCCCTTCAGGCTGTCATCCCATGTT39380.18426822865731257No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39130.18309842019707062No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTC36050.16868637996688973No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG33760.15797093447107344No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG32100.15020340629506687No Hit
GTGTATTACTGTGCGACAGAGTTGAGTGGTGGTGACTCCTACTGGGGCCA31900.14926755952687332No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC31700.14833171275867976No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC31460.14720869663684746No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG30760.14393323294816998No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT30710.14369927125612159No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG30450.14248267045746998No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30050.14061097692108285No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC29660.1387860757231054No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGGAAGTGGGAC29470.13789702129332151No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT29030.1358381584032957No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA28240.13214156366893112No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC27720.12970836207162784No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG27560.12895968465707297No Hit
GAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGGACGGCCCCCAAA27000.126339313706131No Hit
CCCCTGAGGGCCGCTGATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGTTTA26740.12512271290747937No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT25600.11978838632877607No Hit
CCACAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG25350.11861857786853412No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25340.11857178553012443No Hit
GTCCACATAGTGTGTCTTATCTCCATCTTGCTTTATGTTGGCCACCCACT25230.11805706980761799No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT25210.11796348513079863No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25070.11730839239306314No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25060.11726160005465344No Hit
GCCTGAAGGGGTACGTCTTCGGAAGTGGGACCGAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCA24610.11515594482621792No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA24410.11422009805802437No Hit
GGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCA24400.11417330571961469No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC24320.11379896701233727No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC24190.11319066661301146No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24150.11300349725937274No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC23380.10940048720182753No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC22400.10481483803767906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22080.10331748320856936No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21810.10205409007150805No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21760.10182012837945965No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21740.1017265437026403No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG21720.10163295902582095No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG21700.1015393743490016No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21610.1011182433033145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)