FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137102
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69300.3242709051790696No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC68400.3200595947221986No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT67880.31762639312489527No Hit
GATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGG61030.28557364131426577No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC53360.2496839177540426No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC53010.2480461859097039No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCA51500.2409805428098425No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50320.23545904687750047No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG49850.2332598069722456No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC48290.2259602021803358No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA47300.22132776067777765No Hit
GATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGG44250.20705609746282583No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG42500.19886743824113215No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT42090.19694895236633536No Hit
GTCCTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG41540.19437537375380307No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41440.19390745036970627No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT40130.18777765403803845No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC38020.17790447063359632No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37600.17593919242038986No Hit
CTATGTGGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAACGGCAAAGACTCACTGTATCTGCAAATGAA36670.17158750494828978No Hit
GCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCACTTCCGAAGACGTACCCCTTCAGGCTGTCATCCCATGTT33340.15600565625786697No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC33240.15553773287377018No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG32890.15390000102943144No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC32740.15319811595328628No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTC31970.14959510589574104No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG31850.14903359783482492No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT31110.14557096479250875No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG30540.14290380150315707No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG30480.142623047472699No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29680.13887966039992478No Hit
GTGTATTACTGTGCGACAGAGTTGAGTGGTGGTGACTCCTACTGGGGCCA29480.1379438136317312No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA29470.13789702129332151No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGGAAGTGGGAC29120.13625928944898277No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC28380.1327966564066666No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC27370.12807063022728912No Hit
GAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGGACGGCCCCCAAA26860.12568422096839552No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT26820.1254970516147568No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA26780.1253098822611181No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC25990.12161328752675353No Hit
CCCCTGAGGGCCGCTGATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGTTTA24570.11496877547257922No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG23900.11183368879913078No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23830.11150614243026305No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC23300.1090261484945501No Hit
GGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCA23110.10813709406476621No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22920.10724803963498232No Hit
CCACAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG22810.10673332391247586No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA22810.10673332391247586No Hit
GTCCACATAGTGTGTCTTATCTCCATCTTGCTTTATGTTGGCCACCCACT22700.10621860818996942No Hit
GCCTGAAGGGGTACGTCTTCGGAAGTGGGACCGAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCA22590.10570389246746295No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22570.10561030779064359No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22520.1053763460985952No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22360.10462766868404035No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22060.10322389853175001No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGGACGGCCCC21920.10256880579401452No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21800.10200729773309836No Hit
GTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGTTTACAGTATTACTT21740.1017265437026403No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21470.100463150565579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)