FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584643
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT145200.9162947111746935No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC119820.7561324538082079No Hit
GTTTTATACAACTCCAACTACTTAGCTTGGTTCCAACAGAAACCAGGACA103790.6549740225400926No Hit
CTCCTAAACTGCTCATTTACTGGGCGTCTATCCGGGAATCCGGGGTCCCT97470.615091222439376No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAG85350.538607118448761No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71750.45278337139658587No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55180.3482172325249283No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53920.34026591478332974No Hit
GAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT53230.3359116217343591No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA50480.3185575552348384No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT43790.2763398443687316No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43780.27627673867236974No Hit
GTATAAAACACTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACTGCCAGGGAGTCTGGAGA42110.26573808737993354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41280.2605003145818964No Hit
CCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC39610.24996166328946015No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37950.23948611769338585No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37940.23942301199702393No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTT37730.23809779237342418No Hit
GTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTTTATA36900.23286001957538702No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC34920.2203650916957321No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC34640.21859813219759905No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34430.2172729125739993No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA34260.2162001157358471No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC34250.21613701003948524No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33640.2122875625614097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32310.2038945049452779No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31840.2009285372162689No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31430.1983412036654313No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31420.19827809796906937No Hit
GTTTATTACTGTCAGCAGTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA31220.19701598404183152No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT30820.19449175618735576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30250.19089473149472783No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG30110.19001125174566133No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG29920.18881224351478534No Hit
GGCCAAAAGTGTACGGAGTACTATAATACTGCTGACAGTAATAAACTGCC29800.18805497515844263No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29670.187234601105738No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCGTCTATCCGGGAA27500.17354066499520712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27160.17139507131890275No Hit
CTACGGGGACTTCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTG25880.16331754218458036No Hit
GCTCATTTACTGGGCGTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT25730.16237095673915197No Hit
GTCAGGGACCCCGGATTCCCGGATAGACGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAA25570.16136126559736166No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24770.1563128098884102No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTACT24700.15587107001387696No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24310.1534099478557631No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24260.15309441937395363No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24050.15176919975035386No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23850.150507085823116No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23600.14892944341406866No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23280.14691006113048807No Hit
GTGTTTTATACAACTCCAACTACTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTA23020.14526931302507884No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA22620.1427450851706031No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22410.14141986554700334No Hit
GGATCTCTGGTGCCTACGGGGACTTCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGG22070.13927427187069896No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21660.1366869383198613No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC21650.13662383262349942No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21400.13504619021445208No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAGTTGTATAAAACA21280.13428892185810937No Hit
ATCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGC20970.13233264527089067No Hit
CTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTTTATACAACTCCAA20960.13226953957452878No Hit
GTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAGTTGTATAAAACA20850.13157537691454796No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20610.13006084020186248No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20350.12842009209645325No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19920.12570654715289184No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19140.12078430283666416No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA19060.120279457265769No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTAT18870.11908044903489302No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18620.1175028066258457No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18550.11706106675131243No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18300.11548342434226509No Hit
ATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAA18230.11504168446773186No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC17890.11289609079142746No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17460.11018254584786605No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACTTCGTGATGA17440.11005633445514226No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17430.10999322875878037No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC17390.10974080597333279No Hit
GTTCCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCGTCTATCCGGGAATCCGGGGTCCCTGA17280.10904664331335197No Hit
CTGCAAGTCCAGCCACAGTGTTTTATACAACTCCAACTACTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTC17270.10898353761699008No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17270.10898353761699008No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17020.10740589520794273No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16760.1057651471025335No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16540.10437682178257185No Hit
ACTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATT16460.10387197621167671No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16410.10355644772986723No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC16310.10292539076624829No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16120.1017263825353723No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG16040.10122153696447717No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15960.10071669139358204No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15950.10065358569722013No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15920.10046426860813445No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15850.1000225287336012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)