FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584643
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT129300.8159566539592829No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC123000.77620006525129No Hit
GTTTTATACAACTCCAACTACTTAGCTTGGTTCCAACAGAAACCAGGACA87210.5503447779720733No Hit
CTCCTAAACTGCTCATTTACTGGGCGTCTATCCGGGAATCCGGGGTCCCT83710.5282577842454105No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAG76510.48282168286484717No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70070.44218161440778775No Hit
GAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT56080.35389674519749875No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49570.3128149368659061No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT46310.2922424798519288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44600.28145140577404504No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43700.2757718931014746No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA42530.26838852662713303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40790.2574081354601636No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40430.2551363303911354No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36890.2327969138790251No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG34610.21840881510851337No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33740.2129186195250287No Hit
CCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC32540.20534593596160144No Hit
GTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTTTATA32250.20351587076710653No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32120.2026954967144019No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG31280.19739461822000287No Hit
GTATAAAACACTGTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACTGCCAGGGAGTCTGGAGA31130.19644803277457448No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30960.19537523593642228No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30660.19348206504556548No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTT30430.19203063402924192No Hit
GTTTATTACTGTCAGCAGTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA30360.19158889415470864No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA30320.1913364713692611No Hit
GGCCAAAAGTGTACGGAGTACTATAATACTGCTGACAGTAATAAACTGCC29550.1864773327493953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29530.1863511213566715No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC29160.18401621059128143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29080.18351136502038629No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28950.18269099096768168No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28920.182501673878596No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27780.17530762449334014No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGATTCCCGGAT27540.1737930877806547No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT27170.1714581770152646No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27000.17038538017711244No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26810.16918637194623648No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG26370.16640972130631315No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26350.16628350991358937No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26310.1660310871281418No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26120.16483207889726584No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTACT23640.14918186619951623No Hit
GTCAGGGACCCCGGATTCCCGGATAGACGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAA23320.14716248391593564No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC23020.14526931302507884No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22380.14123054845791766No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22300.14072570288702252No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21940.13845389781799433No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21770.13738110097984216No Hit
GCTCATTTACTGGGCGTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT21520.1358034585707948No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCGTCTATCCGGGAA21340.13466755603628072No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21320.13454134464355694No Hit
CTACGGGGACTTCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTG20770.1310705313436528No Hit
GTGTTTTATACAACTCCAACTACTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTGCTCATTTA20630.1301870515945863No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20450.1290511490600722No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20020.1263376041165108No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19930.12576965284925376No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19750.12463375031473965No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19730.12450753892201587No Hit
GGATCTCTGGTGCCTACGGGGACTTCGTGATGACCCAGTCTCCAGACTCCCTGGCAGTGTCTCTGGGCGAGAGGG19670.12412890474384451No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19460.12280368512024475No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19430.12261436803115908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19280.12166778258573066No Hit
GTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAGTTGTATAAAACA19120.12065809144394037No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGTAGTTGGAGTTGTATAAAACA18900.1192697661239787No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG18460.11649311548405539No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGC18440.11636690409133162No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC18410.11617758700224594No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17920.11308540788051313No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17670.1115077654714658No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17250.10885732622426629No Hit
CTCCCTGGCAGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCACAGTGTTTTATACAACTCCAA17000.10727968381521896No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16770.1058282527988954No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTACTCCGT16630.10494477304982888No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16590.10469235026438133No Hit
ATCCGGGAATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGC16500.10412439899712427No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTC16410.10355644772986723No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16370.10330402494441966No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16240.10248365089171504No Hit
TCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTAT16230.10242054519535315No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16230.10242054519535315No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTGTACGGAGTA16180.10210501671354369No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACTTCGTGATGA16160.10197880532081989No Hit
ATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAA15980.10084290278630581No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15950.10065358569722013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)