FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362347
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45880.33677176226027583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45250.33214738976193287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38440.28216012513698785No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32190.23628341384390322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32040.23518237277286919No Hit
GTCTATGGTGGGTCCTTCAGTGGTTCCTTCTGGAGCTGGATCCGCCAGTC30520.224025156586391No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29500.21653807730335956No Hit
GTAATATACAGCCGTGTCCGCGACGCTCACAGAGCTCAGCTTCAGGGAGA27480.2017107242134346No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27320.20053628040433163No Hit
GATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA27210.19972885028557336No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGCCTATTTAAATTGGTATCAGCAAAAA27020.19833419826226356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26070.1913609381457147No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25750.18901205052750877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25350.18607594100475136No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24560.18027712469730545No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24380.17895587541206462No Hit
GTCTCCATCCTCCCTGACTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCGC23680.17381768374723916No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23090.16948692220117195No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATT22130.16244025934655415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21940.16104560732324436No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21800.16001796899027929No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21570.15832970601469376No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC21070.15465956911124698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20730.15216387601690318No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20380.14959478018449043No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19290.14159388173497647No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18830.13821735578380545No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18640.13682270376049568No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18630.13674930102242674No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGCA18370.13484082983263443No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18360.1347674270945655No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18330.13454721888035867No Hit
CTCCTGATCTATGCTGCGTCCACTTTGCAAAGTGGGGTCCCACCAAGGTT18150.13322596959511784No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18040.13241853947635954No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17970.13190472030987702No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17970.13190472030987702No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17620.12933562447746427No Hit
GGGTTCCAGTGCCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT17110.12559208483594855No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16650.12221555888477752No Hit
GTATATTACTGTGCGAGGAGGGCTAGTGGCTCCGTCCGCTTCCACTTCTA16620.12199535067057071No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16420.120527295909192No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAT16100.11817840829098608No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16060.11788479733871034No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15620.11465507686367717No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGGCACTTACTACTGTCAACAGGGTTCCAGTGCC15570.1142880631733325No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15530.11399445222105675No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15390.11296681388809164No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15220.11171896734091975No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15090.11076473174602358No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14950.1097370934130585No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14840.1089296632943002No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14630.10738820579485255No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14480.10628716472381854No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14240.10452549901016407No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14230.10445209627209515No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14130.10371806889140579No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14020.1029106387726475No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13970.10254362508230282No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13950.10239681960616494No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13840.10158938948740667No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13760.10100216758285518No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13730.10078195936864837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4100.051.487341
GTCGTAC200.007753128751.072121
GTCGTAT306.3591567E-445.93033639
TCGTATG2400.043.11574640
TATCTCG2150.041.50357436
CGTATGC2750.041.44970341
GCCGTCT2600.041.43712646
AGCCTAT3300.040.4301332
CTTATAC3550.040.2822381
GAGACCG3450.039.49198524
ACGAGAC3450.039.4832822
ATCTCGT2200.039.0436537
ACCGGAG3500.038.9621627
AGACCGG3500.038.93925525
GCCTATC2650.038.76847533
GTATCAA41500.038.7245641
CCGTCTT2800.038.5379947
TATACAC3800.038.5238463
GACCGGA3550.038.3978726
GAGCCTA3700.037.88055431
Produced by FastQC (version 0.11.5)