FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641963
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATAGTGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAATAGCAATCGG54550.33222429494452677No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44940.27369678853908397No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA44820.27296595599291823No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39840.24263640532703845No Hit
GATTATTACTGTCAGGCCTATGACAGCAGCCTAAGTGGTTCGGTTTGGGT38010.2314912089980103No Hit
CTATAACCTGCCCCGATGTTGGAATTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA37820.23033405746658114No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAACCGAACCA36440.22192948318567474No Hit
CTCCTGCACTGGGAGCAATTCCAACATCGGGGCAGGTTATAGTGTACACTGGTATCAGCAGCTTCCAGGAACAGC35760.21778809875740196No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34470.20993164888611984No Hit
GTCATAGGCCTGACAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG31240.1902600728518243No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG30820.18770215894024408No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30810.1876412562280636No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30490.1856923694382882No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28760.1751562002310649No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28000.1705275941053483No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT26990.16437642017511966No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26330.16035684117120788No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26050.1586515652301544No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25360.15444927808970116No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25140.15310941842173056No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25040.15250039129992576No Hit
GTGATGGCCAGGGAGGCTGACGTGCCGGACTTGGAGCCAGAGAATCGGTC24830.15122143434413565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24280.14787178517420915No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGG24020.14628831465751663No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23750.14464394142864365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23640.14397401159465834No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23610.1437913034581169No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23390.14245144379014632No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG22610.13770103224006874No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21840.13301152340217168No Hit
AGGTTATAGTGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAAC20810.12673854404758209No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20360.12399792199946041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20330.12381521386291895No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20050.12210993792186547No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19800.12058737011735343No Hit
GGATGAGGCTGATTATTACTGTCAGGCCTATGACAGCAGCCTAAGTGGTTCGGTTTGGGTGTTCGGCGGAGGGAC19760.12034375926863151No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19150.11662869382562213No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGTCAGGCCTAT19120.11644598568908068No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAATTCCAACATCGGGGCAGGTTATAGTGTACACTGGTATCAG19070.1161414721281783No Hit
GCCTAAGTGGTTCGGTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT18500.11267001753389083No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18430.11224369854862747No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17940.10925946565178386No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC17900.10901585480306195No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17790.10834592496907665No Hit
GCCTATGACAGCAGCCTAAGTGGTTCGGTTTGGGTGTTCGGCGGAGGGAC17660.1075541897107304No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17520.10670155174020365No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17520.10670155174020365No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17470.10639703817930124No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17350.10566620563313546No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAA16860.10268197273629186No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16680.1015857239170432No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC16660.10146391849268223No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16500.10048947509779453No Hit
GTGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATC16460.10024586424907261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG552.8503564E-949.66100734
GCGAATC2750.042.1562333
CGTATGC1950.040.6819641
GTGGTAT5350.039.9105951
AATCGCG350.001400810939.12140736
ACGAGAC3650.038.0627222
GGCGAAT3700.037.74955732
TAAGGCG3800.037.55834229
GTATCAA41250.037.548591
ATGCCGT2050.037.22848544
CGAGACT3750.037.04884323
TCGTATG2050.036.9531140
ACTAAGG3800.036.61153827
AAGGCGA3950.036.15526230
TCTCGTA1900.036.1251338
ATCTCGT1900.036.079537
TCTCCGA3800.035.663111
GACTAAG3950.035.1986826
AGACTAA4050.034.31702825
CCTAGTA400.002834310833.8892332
Produced by FastQC (version 0.11.5)