FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234417
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41360.33505695401148883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40530.32833313215874377No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40260.3261458648090556No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34990.2834536465392165No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32330.2619050126496962No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30720.24886241845340756No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30640.24821433923868513No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27600.22358732907923334No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG26730.21653946761912707No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24130.1954768931406486No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23750.1923985168707171No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23420.18972519010998715No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA23310.18883408118974382No Hit
GATTATTACTGCAGCTCATATGGAGGCAGCAACTGTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAG22990.18624176433085415No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC22710.1839734870793257No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22680.1837304573738048No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22000.17822178404866426No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21500.17417128895664918No Hit
GACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACACAGTTGCTGCCTCCATATGAGCTGCAGTAATAATCAG21230.17198402160696102No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21100.1709308928830371No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGGAGGCAGCAACTGTGTCTTCGGAA20870.16906766514071014No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTCATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC19640.15910344721435302No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACACAGTTGCTGC19500.1579693085885888No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTCATAACTATGTCTCCTGGTACCAACAACA18200.14743802134934955No Hit
GTCTATAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT18120.14678994213462712No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18040.1461418629199047No Hit
ACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACACAGTTGCTGCCTCCATATGAGCTGCAGTAATAATCAGC17800.14419762527573746No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17370.14071419949660446No Hit
ATATTACTGTGCGAAGGACTATCCACAACACCTGGTACGTCGATACTTTGACTACTGGGGCCAGGGAACCCTGGT16620.13463845685858183No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16560.13415239744754No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16380.1326942192144146No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16380.1326942192144146No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16220.13139806078496974No Hit
CATATGGAGGCAGCAACTGTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCA16150.13083099147208765No Hit
CTATATTACTGTGCGAAGGACTATCCACAACACCTGGTACGTCGATACTT15670.12694251618375316No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15190.12305404089541865No Hit
CCCCTGAGGGCCGCTTATAGACCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14710.11916556560708416No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATA14360.1163302190426736No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14310.11592516953347208No Hit
CTTATAGACCTCAAAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTT14220.11519608041690936No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14160.11471002100586754No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14150.11462901110402723No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTTTGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14010.11349487247826302No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13970.11317083287090181No Hit
GCCTTGGGCTGACGTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACACA13830.11203669424513758No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGGAGGCAGCAACTGTGTCTTCGGAACTGGGACCAAGGTCACCGT13560.10984942689544944No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13480.109201347680727No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13470.1091203377788867No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13300.10774316944760158No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13240.10725711003655976No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13170.10669004072367766No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13010.10539388229423281No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13000.10531287239239254No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12880.10434075357030889No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT12760.10336863474822529No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12760.10336863474822529No Hit
TCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12750.10328762484638498No Hit
TAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTTTGAGGTCTATAAGCGGCC12630.10231550602430135No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12550.10166742680957895No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG12540.10158641690773865No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12360.10012823867461319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)