FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550018
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52250.33709285956679214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46440.2996094238905613No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45970.2965772010389557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35570.22948120602470423No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34840.22477158329774236No Hit
GTTTTACACACGCCGGACAATAAGAACTACTTAGCTTGGTATCAGCAGAA33740.2176748915173888No Hit
GTTTATTACTGTCAGCATTATCTTCGTGTTCCCGTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA33390.21541685322363996No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33300.21483621480524745No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32560.21006207669846413No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31870.20561051549078785No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31360.20232023111989667No Hit
AGCCAGGACAGCCTCCTAAACTTCTCATTTCCTGGGCATCTGCCCGGGAA30630.1976106083929348No Hit
CTCCTAAACTTCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGATCCCT29490.1902558550932957No Hit
GTCTGAAGACGTGGCAGTTTATTACTGTCAGCATTATCTTCGTGTTCCCGTAACTTTTGGCCAGGGGACCAAG28890.18638493230401196No Hit
GTTTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACGTGGCAGTTTATTACTGTCAGCATTATCTTCGTGTT28870.18625590154436916No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28710.18522365546722683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28010.18070757887972913No Hit
GTAATAAACTGCCACGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAACTGTCCCAGACCCGCTGCCA27970.18044951736044357No Hit
GTTTAGGAGGCTGTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTCCGGCGTGTGTAAAACACT26970.173997979378304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25770.16625613379973653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25370.1636755186068807No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25280.16309488018848814No Hit
GTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTCCGGCGTG24480.1579336498027765No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23600.15225629637849367No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22670.1462563660551039No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21580.13922418965457176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT21580.13922418965457176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21450.1383854897168936No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21010.13554681300475221No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA19990.12896624426296985No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19810.12780496742618475No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19780.12761142128672054No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19140.12348243697815123No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19070.12303082931940144No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18940.1221921293817233No Hit
GTGTAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18720.1207727910256526No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACACACGCCGGA18470.11915990653011772No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18440.11896636039065353No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18350.11838572197226097No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18270.1178695989336898No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17430.11245030702869258No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGTTTTCACTCTCACCATCAGCAGCC17400.1122567608892284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17250.11128903019190745No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17110.1103858148744079No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16820.10851486885958743No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16440.10606328442637442No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16120.10399879227208973No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16070.10367621537298276No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15920.10270848467566183No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15640.10090205404066274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG306.4917526E-445.73879236
GTATCAA47550.039.4482151
CGTATGC2300.039.0668741
GTGGTAT5700.036.4318541
TCGTATG2200.036.06387340
TAAGGCG3500.035.1061529
GCGAATA701.0357307E-634.2295333
ATCAACG55950.033.64633
TATCAAC55750.033.6448822
TCAACGC56300.033.5580644
ATGCCGT2600.033.36547544
GGCGAAT3600.033.2463532
CAACGCA57550.032.828115
AACGCAG57900.032.5708776
ACTAAGG3800.032.30430627
CACGAGA3750.031.78155921
CGAATAT553.3977852E-431.13896834
GACTAAG3950.031.06551226
CTTATAC4100.030.7216221
CGAGACT4250.030.45406223
Produced by FastQC (version 0.11.5)