FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550018
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45710.2948998011635994No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45540.2938030397066357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40530.26148083441611647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35590.229610236784347No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32400.2090298306213218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31070.2004492851050762No Hit
GTAATAAACTGCCACGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAACTGTCCCAGACCCGCTGCCA30660.19780415453239897No Hit
GTTTATTACTGTCAGCATTATCTTCGTGTTCCCGTAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA30130.19438483940186502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29840.19251389338704455No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29770.19206228572829476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28810.18586880926544078No Hit
GTTTTACACACGCCGGACAATAAGAACTACTTAGCTTGGTATCAGCAGAA27890.17993339432187241No Hit
GTTTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACGTGGCAGTTTATTACTGTCAGCATTATCTTCGTGTT27420.1769011714702668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26890.17348185633973284No Hit
GTCTGAAGACGTGGCAGTTTATTACTGTCAGCATTATCTTCGTGTTCCCGTAACTTTTGGCCAGGGGACCAAG26890.17348185633973284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26770.17270767178187607No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26420.17044963348812722No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26370.17012705658902025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26050.16806256443473558No Hit
CTCCTAAACTTCTCATTTCCTGGGCATCTGCCCGGGAATCCGGGATCCCT25580.16503034158313No Hit
GTTTAGGAGGCTGTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTCCGGCGTGTGTAAAACACT24000.1548369115713495No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT23140.14928858890670946No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23120.1491595581470667No Hit
GAGTGAAAACTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATCCCG22770.14690151985331784No Hit
AGCCAGGACAGCCTCCTAAACTTCTCATTTCCTGGGCATCTGCCCGGGAA22690.14638539681474666No Hit
GTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTCCGGCGTG22120.14270802016492712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21750.14032095111153547No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21600.13935322041421455No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21590.13928870503439314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20340.1312242825567187No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19990.12896624426296985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19750.12741787514725636No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19470.12561144451225728No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19410.12522435223332892No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19340.12477274457457913No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATCCCGGATTCCCGGGC18580.11986957570815307No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18580.11986957570815307No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAACTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGATC17510.11296643006726374No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17330.11180515323047861No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17300.11161160709101442No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17270.11141806095155023No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17030.10986969183583674No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTACA16490.10638586132548139No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15910.10264396929584042No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15670.10109560018012693No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15530.10019238486262741No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGTTTTCACTCTCACCATCAGCAGCC15520.10012786948280601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)