FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566270
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25030.44201529305808185No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17300.30550797322831863No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGAACAGAATTCT14490.2558850018542391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13120.23169159588182314No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12100.2136789870556448No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11780.20802797252194183No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11290.1993748565172091No Hit
GCATAATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11040.19496000141275363No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10650.1880728274498031No Hit
ACATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGAGAATTCTGTT10410.18383456654952585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9230.16299645045649602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9000.15893478376039696No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGAGAATTCTGTTCCAGATCCACTGCCG8950.1580518127395059No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA8800.1554028996768326No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8780.15504971126847616No Hit
GCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGC8590.15169442138909No Hit
ATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA8570.15134123298073357No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8520.1504582619598425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8350.14745616048881274No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8230.14533703003867413No Hit
ACTTATTACTGTCTACAGCATAATGTTTATCCTCAGACTTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA8130.14357108799689194No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7750.13686050823811963No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7680.13562434880887209No Hit
AGTCAGGACATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGTCCCTAAGCGCCTGATCTATG7640.1349179719921592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7540.13315202995037703No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7130.12591166757907005No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7080.12502869655817897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6970.12308616031221856No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6970.12308616031221856No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6900.12185000088297102No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6850.12096702986207993No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6820.12043724724954527No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAAAGTC6800.12008405884118883No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6630.1170819573701591No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6610.11672876896180266No Hit
CAGTAATACATGGCCGTGTCGTCAGATTTCAGGCTGCTCAGCTCCATGTA6540.11549260953255513No Hit
CTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATTGCTAA6150.10860543556960461No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6120.10807565295706996No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6060.10701608773200062No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5820.10277782683172339No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGAACAGAATTCTCT5800.10242463842336695No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5740.10136507319829763No Hit
GTCCCTAAGCGCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT5700.10065869638158476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTCG100.008035244139.0425970
CTAAACG200.002758406666.2992569
TTCGAGT200.006749558352.89013756
CTCTAGT200.007613688751.30319631
GTGGTAT2450.047.2711521
AATGGTA2800.039.72644870
GTATCAA19700.038.3857571
CGGTACC501.7919994E-434.71992545
TTAACGG501.9350927E-434.26905435
TTATACC400.002776994834.0269364
TATCAAC22750.033.371562
ATCAACG23400.032.5785453
TCAACGC23600.032.152574
TATTAGA851.355711E-732.042332
TAACCGT552.9185298E-431.9522853
GTATTAG751.8416758E-631.7921681
TCCGCGG5500.031.60058870
CAACGCA24050.031.5509645
AACGCAG24200.031.3553986
ATGAATC450.004943539430.24616212
Produced by FastQC (version 0.11.5)