FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712544
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAG82230.4801628454509782No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT81520.4760169665713698No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG69070.4033181045275333No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG63130.36863286432348596No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGACTGCAAAGGAGCAGCAGTAATACCCAG61730.36045789188482164No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC60920.35572808640245157No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60320.3522245267858811No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC59530.34761150662406337No Hit
GTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGTAGG59080.3449838369116356No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC56790.3316119177083917No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG56200.32816675075209745No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC49320.2879926004820898No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC48330.28221172711474857No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG48230.2816278005119868No Hit
GTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCC46010.26866462993067625No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATA45920.2681390959881907No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGACTGCAAAGGAGCAGCAGTAATACCCAGC44060.2572780611768223No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC43540.25424164284246126No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT43060.2514387951492049No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42080.2457163144421399No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAG42020.24536595848048282No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGA42010.24530756582020666No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT41880.24454846123661642No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG40530.23666545209933293No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACA40410.23596474017601884No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG40010.2336290337649719No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39990.23351224844441956No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC39990.23351224844441956No Hit
CCCCTGAGGGCCGCTGATCGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA38330.22381906683857467No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG38040.2221256796905656No Hit
GCAGTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGT37850.2210162191453183No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35940.2098632210325691No Hit
GTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACACCCAGGC35720.20857858250649328No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG33440.1952650559635256No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC33060.193046134873031No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAACTTATGA32770.19135274772502195No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG32100.18744043948651828No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC30900.1804333202533774No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT29950.1748860175271409No Hit
CCTCAGGGGTCCCTGATCGCCTCTCTGGCTCCAAGTCTGGCAACACGGCCTACCTGACCATCTCTGGGCTCCAGG29600.17284227441747482No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA29560.17260870377637014No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTG29220.17062335332698023No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC28680.16747014967206683No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28480.16630229646654335No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG28430.16601033316516248No Hit
GCCCAGAGATGGTCAGGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATCAGGGACCCCTGAGGGCCG28040.1637330194143917No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC27990.16344105611301082No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT27870.16274034418969674No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC26070.15222966533998541No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT25900.15123699011529046No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT24780.14469701216435898No Hit
CCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGG23400.13663882504624697No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC23290.13599650578320907No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC23240.1357045424818282No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA23130.1350622232187903No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG23100.13488704523796177No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23070.13471186725713324No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG23020.13441990395575237No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT22320.1303324177364202No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG22070.12887260122951585No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC22010.12852224526785883No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21860.12764635536371619No Hit
GAGATGGTCAGGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATC21810.12735439206233534No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT21760.12706242876095447No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC21650.12642010949791654No Hit
CCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA21530.12571939757460246No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG21360.1247267223499075No Hit
GCCTACCTGACCATCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTA21220.12390922510604108No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT21020.12274137190051758No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG20610.12034727282919447No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20370.11894584898256629No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20030.11696049853317637No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC19780.11550068202627202No Hit
GGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATCAGGGACCCCT19770.11544228936599585No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA19590.11439122148102472No Hit
CAGTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGTA19430.11345693891660594No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG19330.11287301231384421No Hit
CTGCAAAGGAGCAGCAGTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGTAGGCCGTGTTGC19130.11170515910832071No Hit
GCCCCCAAACTCATCATTTATGATGTCGATCAGCGGCCCTCAGGGGTCCCTGATCGCCTCTCTGGCTCCAAGT18780.10966141599865463No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG18470.10785124353009325No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18430.10761767288898853No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18380.10732570958760768No Hit
GCCCTCAGGGGTCCCTGATCGCCTCTCTGGCTCCAAGTCTGGCAACACGG18220.10639142702318889No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18140.1059242857409795No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA17960.10487321785600838No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC17850.10423089859297047No Hit
GCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCC17700.10335500868882785No Hit
GACCATCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTT17670.10317983070799934No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTT17540.10242072612440907No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG17540.10242072612440907No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17520.10230394080385673No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG17480.10207037016275201No Hit
GGCAAAGCCCCCAAACTCATCATTTATGATGTCGATCAGCGGCCCTCAGG17370.10142805089971411No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG17340.1012528729188856No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17200.10043537567501915No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17150.10014341237363827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4250.049.41755370
GGTATCA13400.044.6706921
GTGGTAT3400.042.253221
TGGTATC3550.041.4289742
GTATCAA33700.039.0769161
CGTATGC5000.037.97147841
TATACAC5900.037.6823583
ATATCGT655.586535E-737.03950537
TCTCGTA4000.037.00596638
TCGTATG5150.036.836240
TTATACA6050.036.7470132
CTTATAC6150.036.1516151
ATGCCGT5500.035.90919544
ATCTCGT4050.035.66767537
ACGAGAC6050.035.61425822
AGGCAAT5700.035.49950832
AATCTCG4100.035.2058236
GGCAATC4450.034.70689433
ATCGTAT709.822743E-734.46264639
TATCAAC38650.034.4242252
Produced by FastQC (version 0.11.5)