FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712544
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAG79010.4613604088420502No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT70940.4142375319991778No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC65070.37996104041706374No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG61930.3616257450903451No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC59640.3482538258871013No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG56870.33207905899060114No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGACTGCAAAGGAGCAGCAGTAATACCCAG56210.32822514341237363No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG55930.3265901489246408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55860.32618140030270754No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49940.29161294541921257No Hit
GTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGTAGG49690.29015312891230827No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC48300.28203654913392007No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG46760.2730440794513893No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC45680.2667376721415625No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44680.2608984061139451No Hit
GTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCC44550.26013930153035486No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG43050.25138040248892873No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT42830.25009576396285293No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC42640.24898630341760564No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATA41880.24454846123661642No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC41180.2404609750172842No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGA40330.23549759889380947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39780.23228600257861987No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTTCGGAG39540.2308845787319917No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACA38350.223935852159127No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGGCATAAGAGCCGACTGCAAAGGAGCAGCAGTAATACCCAGC38320.22376067417829848No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG37920.22142496776725154No Hit
CCCCTGAGGGCCGCTGATCGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA37190.21716230356709082No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT36520.21324999532858716No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35760.20881215314759796No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG34890.20373199170357084No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG34570.20186342657473327No Hit
GTGATATTGGTGCTCATAAGTTTGTCTCCTGGTACCAACAACACCCAGGC34280.2001700394267242No Hit
GCAGTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGT33740.1970168357718108No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG32070.18726526150568978No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAACTTATGA31920.18638937160154717No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG31570.18434562849188108No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC30450.1778056505409496No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA30340.1771633312779117No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC28880.1686380028775903No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTG28490.1663606891268195No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT28180.16455051665825812No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC27260.15917839191285013No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC27230.1590032139320216No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26870.15690107816207932No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT26570.15514929835379412No Hit
CCTCAGGGGTCCCTGATCGCCTCTCTGGCTCCAAGTCTGGCAACACGGCCTACCTGACCATCTCTGGGCTCCAGG25490.14884289104396733No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG25450.14860932040286265No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT24870.14522254610684454No Hit
GCCCAGAGATGGTCAGGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATCAGGGACCCCTGAGGGCCG24720.14434665620270193No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC24070.14055113328475063No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC24020.14025916998336976No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT23110.13494543789823796No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT22960.13406954799409534No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22890.1336607993721621No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22190.12957331315282994No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT22170.1294565278322776No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21520.12566100491432627No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG21430.12513547097184072No Hit
CCAATATCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGG21200.12379243978548873No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21040.12285815722106995No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20580.12017209484836593No Hit
GCCTACCTGACCATCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTA20460.11947138292505186No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG20140.11760281779621429No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT20090.11731085449483343No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC20050.11707728385372872No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC19940.1164349645906908No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19630.1146247921221294No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19590.11439122148102472No Hit
CTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTC19460.11363211689743445No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19410.1133401535960536No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19350.11298979763439655No Hit
GAGATGGTCAGGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATC19090.11147158846721603No Hit
GGTAGGCCGTGTTGCCAGACTTGGAGCCAGAGAGGCGATCAGGGACCCCT18850.11007016462058784No Hit
CCTTTGCAGTCGGCTCTTATGCCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA18750.10948623801782612No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG18710.10925266737672143No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC18380.10732570958760768No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18140.1059242857409795No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA18120.10580750042042716No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG17860.10428929125324664No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA17690.10329661602855168No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGC17550.10247911878468524No Hit
GACCATCTCTGGGCTCCAGGCTGACGATGAGGCTGGGTATTACTGCTGCTCCTTTGCAGTCGGCTCTTATGCCTT17490.10212876282302819No Hit
CTGCAAAGGAGCAGCAGTAATACCCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGTAGGCCGTGTTGC17470.10201197750247584No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGCTCATAAGTT17390.10154483622026646No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC17380.10148644355999029No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA17340.1012528729188856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)