FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243301
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35430.2849671961978636No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32050.25778150262888877No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30360.2441886558444013No Hit
CTATTGGAGACCAAGCAATAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC29850.2400866724952365No Hit
GTACTGGAGCTGTCACCGATGGTCATAATCCCTACTGGTTCCAGCAGAAG29170.23461736136301667No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGGACCACTATTGGAGACCAAGCAATAATAGTCAG26450.21274011683413752No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26280.2113727890510826No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCA25820.207672960932228No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25660.20638606419523509No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25130.20212321875394615No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24360.19593002820716787No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23690.1905411481210101No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23600.18981726870645163No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23090.18571528535728676No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22740.18290019874511482No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22450.1805676984093152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20890.1680204552236345No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20700.16649226534845543No Hit
GTCATAATCCCTACTGGTTCCAGCAGAAGCCTGGCCAGGCCCCCAGGACA20560.16536623070358666No Hit
CTCCAATAGTGGTCCCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCC20260.162953299321725No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19680.15828829865012575No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19560.15732312609738108No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC19000.15281898751790596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18600.14960174567542373No Hit
GCCTGAAGATGAGGCTGACTATTATTGCTTGGTCTCCAATAGTGGTCCCTGGGTGTTCGGCGGAGGGACCAAA18570.14936045253723756No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18540.14911915939905138No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17500.1407543306085976No Hit
TCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGAGAGCCTGAGAACCGGGCAGGGGTC17430.14019131328616322No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA17250.1387435544570462No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17050.13713493353580508No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16920.13608932993699835No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT16840.1354458815685019No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16410.1319873465878335No Hit
GTTCTCAGGCTCTCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG16300.1311026050811509No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15390.12378337988950383No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15210.12233562106038683No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCTCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC15200.12225519001432476No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14850.1194401034021528No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14790.11895751712578048No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14360.11549898214511208No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13980.11244260239475397No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13910.11187958507231958No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13600.10938622264439586No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGAGAGCCTGAGAACCGGGCAGGGGTCCAGGAATGTTTGTTG13550.10898406741408556No Hit
GGGCAGCTTTGCCCCCAAGGAGAGAGCCTGAGAACCGGGCAGGGGTCCAG13470.10834061904558911No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13430.10801889486134089No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA12860.1034343252358037No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12700.10214742849881082No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12670.10190613536062466No Hit
CTATTATTGCTTGGTCTCCAATAGTGGTCCCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCC12450.10013665234725944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)