FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243301
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34760.27957831611170586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34720.27925659192745766No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31720.2551272781088409No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28490.2291480502307969No Hit
GTACTGGAGCTGTCACCGATGGTCATAATCCCTACTGGTTCCAGCAGAAG26730.21499218612387508No Hit
CTATTGGAGACCAAGCAATAATAGTCAGCCTCATCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC26610.21402701357113038No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24840.19979071841814655No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24420.1964126144835402No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCA23580.1896564066143275No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCAGGGACCACTATTGGAGACCAAGCAATAATAGTCAG23480.18885209615370693No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23160.18627830267972115No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23140.18611744058759705No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21740.17485709413890924No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21730.17477666309284717No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21060.16938778300668944No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA20610.16576838593389695No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20190.1623902819992906No Hit
CTCCAATAGTGGTCCCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCC19480.15667967772888464No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19090.15354286693246447No Hit
GTCATAATCCCTACTGGTTCCAGCAGAAGCCTGGCCAGGCCCCCAGGACA18160.14606277964869327No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17710.14244338257590078No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17620.14171950316134227No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17530.14099562374678376No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17480.14059346851647347No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17400.13995002014797703No Hit
GCCTGAAGATGAGGCTGACTATTATTGCTTGGTCTCCAATAGTGGTCCCTGGGTGTTCGGCGGAGGGACCAAA17020.13689364039761892No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT16130.12973527729809595No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC16000.12868967369928924No Hit
GTTCTCAGGCTCTCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG15950.12828751846897896No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15910.1279657942847307No Hit
TCTTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGAGAGCCTGAGAACCGGGCAGGGGTC15460.12434639721193821No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15150.12185303478401449No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCTCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC15050.12104872432339393No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14970.12040527595489749No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14800.11903794817184254No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14330.11525768900692591No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC14020.11276432657900219No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13920.11196001611838163No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13860.11147742984200928No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13570.10914492950620967No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13380.10761673963103062No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13320.10713415335465827No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13250.10657113603222389No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13250.10657113603222389No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12870.10351475628186577No Hit
GGGCAGCTTTGCCCCCAAGGAGAGAGCCTGAGAACCGGGCAGGGGTCCAG12700.10214742849881082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)