FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416158
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACAGGTTGTGTGGCCTCCTATGAGGTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAG45410.3206563109483546No Hit
CCTATGAGGTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG38200.26974391275549764No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28090.19835357354193528No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACTGCTGATGTCCCACACCTGACAGTAGTAGTCAC27660.19531718918369279No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25750.18182999354591792No Hit
GCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCC24080.1700375240615807No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC23940.16904893380540872No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23070.16290555149919714No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC22880.16156389329439227No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22610.1596573263717749No Hit
ATACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTTTACTT22370.15796260021833722No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTG21570.1523135130402116No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTGTGGCCTCCTATGAGGTGACT20820.14701749381071885No Hit
GTATTACTGTGCCAAAGGCCCAGATACAGGAATGCTCCCTATTGACTATTGGGGCCAGGGAACCCTGGTCACCGT19540.1379789543257179No Hit
CAGTAATACAAGGCGGTGTCCTCAGTGCTCAGACTGTTCATTTGAAGATG19000.1341658204804831No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTA18980.13402459330102998No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18950.13381275253185027No Hit
CCCCTGTATTGGTCATCTATAGGGATATTAAGGGGCCCTCTGGGATCCCT18620.13148250407087345No Hit
GGCCAGGCCAGGCCCCTGTATTGGTCATCTATAGGGATATTAAGGGGCCC18130.1280224381742715No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18070.1275987566359121No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18010.12717507509755266No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCAGCAGCTCTAGTCTCC17510.12364439561122417No Hit
CTCTTATTACTTGGGATGGTGGTAAGACATCCTATGCAGACTCTGTGAAG17510.12364439561122417No Hit
GTATTGGTCATCTATAGGGATATTAAGGGGCCCTCTGGGATCCCTGAGCG17310.12223212381669278No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17040.12032555689407537No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16940.11961942099680968No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16650.11757162689473916No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCAGCAG15830.1117813125371604No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15780.11142824458852756No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15630.110369040742629No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15590.11008658638372272No Hit
GTCTGAGCACTGAGGACACCGCCTTGTATTACTGTGCCAAAGGCCCAGAT15380.10860370099946474No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15350.10839186023028503No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15320.10818001946110534No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15200.1073326563843865No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15070.10641467971794108No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14910.10528486228231594No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14860.1049317943336831No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14640.10337829535969857No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCA14480.10224847792407343No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTGTGG14480.10224847792407343No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14390.10161295561653431No Hit
TCTATAGGGATATTAAGGGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAACTCGGGGAACACGGCCACC14230.1004831381809092No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14170.10005945664254977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT451.8718802E-645.96358539
AGGGGGG4150.045.8528370
TGGTATC4750.041.0268332
GTGGTAT4600.040.8810731
CTAGCGC5050.038.63598628
CGAGACT5400.037.9904923
ACGAGAC5500.037.9160622
ATGCCGT4300.037.8858644
TATACAC5600.037.8534743
TCGTATG3850.037.65215740
GTATCAA37000.037.6105841
TAGCGCT5200.037.53343229
GGTATCA14050.037.476741
CGTATGC4150.037.4613941
ACTAGCG5300.037.44482427
CTTATAC5950.036.777351
TCTCGTA2950.036.18816838
CACGAGA5650.035.69803621
GACTAGC5800.035.38545626
CCCACGA6000.034.18419619
Produced by FastQC (version 0.11.5)