FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416158
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49020.34614781683964646No Hit
CTTTACAGGTTGTGTGGCCTCCTATGAGGTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAG39400.278217543522686No Hit
CCTATGAGGTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG31550.22278587558732854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26960.1903742379028329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25850.18253612944318362No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACACTGCTGATGTCCCACACCTGACAGTAGTAGTCAC24220.17102611431775266No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21640.15280780816829762No Hit
ATACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTTTACTT21020.14842976560525026No Hit
GCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCC20960.14800608406689084No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC20850.14722933457989856No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC20440.14433417740110918No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTGTGGCCTCCTATGAGGTGACT20210.1427100648373981No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTG20140.1422157697093121No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20060.14165086099149954No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCA18470.1304233002249749No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18210.1285873468920841No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18030.1273163022770058No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCAGCAG17650.12463298586739616No Hit
CTCTTATTACTTGGGATGGTGGTAAGACATCCTATGCAGACTCTGTGAAG17600.1242799179187633No Hit
GTATTACTGTGCCAAAGGCCCAGATACAGGAATGCTCCCTATTGACTATTGGGGCCAGGGAACCCTGGTCACCGT17540.12385623638040388No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGCATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTA17450.12322071407286474No Hit
CCCCTGTATTGGTCATCTATAGGGATATTAAGGGGCCCTCTGGGATCCCT17250.12180844227833335No Hit
GGCCAGGCCAGGCCCCTGTATTGGTCATCTATAGGGATATTAAGGGGCCC16300.1151001512543092No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCAGCAGCTCTAGTCTCC16190.11432340176731692No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16160.11411156099813721No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15910.11234622125497296No Hit
CAGTAATACAAGGCGGTGTCCTCAGTGCTCAGACTGTTCATTTGAAGATG15680.11072210869126184No Hit
GTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15480.10930983689673045No Hit
GTCTGAGCACTGAGGACACCGCCTTGTATTACTGTGCCAAAGGCCCAGAT15400.10874492817891789No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14800.10450811279532368No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTGTGTGG14790.10443749920559711No Hit
GTGGTAAGACATCCTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCC14190.10020068382200291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.043.6082651
GGTATCA16800.042.903091
TGGTATC4900.041.1137542
GTATCAA41300.037.302841
TATCAAC45500.033.9201852
ATCAACG47750.032.3024443
TCAACGC48450.031.826754
CAACGCA49650.031.1949485
AACGCAG49450.031.1831366
GTACGGA450.004877866730.33210214
ACGCAGA51900.029.71117
CGCAGAG52350.029.26028
AGAGTAC53500.028.75776111
CAGAGTA54600.028.24186910
GCAGAGT55150.028.0220789
AATGGTA6800.027.01032870
GACAGGT2800.023.179871
GAGTACT19350.022.9213812
AGTACTT19650.022.4009713
TGACGAA800.00259583821.97772456
Produced by FastQC (version 0.11.5)