FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540284
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55880.36279023868325583No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43080.27968868078873765No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39750.25806929111774196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39500.2564462138151146No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTGTGGTCCCTCCTCCAAATTTGAAAGAGTATGACCCTGCATATGAGCAG37680.2446302110519878No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34100.22138774407836478No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31540.20476743249946114No Hit
GATTATTACTGCTGCTCATATGCAGGGTCATACTCTTTCAAATTTGGAGGAGGGACCACACTGACCGTCCTGGGT30320.19684681526263986No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30290.19665204598632458No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30030.1949640455915922No Hit
GTGTGGTCCCTCCTCCAAATTTGAAAGAGTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAG29470.19132835243370702No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29430.19106866006528667No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTGTGGTCCCTC28000.18178465789425846No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27800.18048619605215663No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAC27310.1773049645390071No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27250.17691542598637655No Hit
TCTCTGGGCTCCAGGCTGAAGATGAAGCTGATTATTACTGCTGCTCATAT25900.16815080855218908No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25390.16483973085482936No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24420.1585421909206354No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24040.15607511342064193No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23670.1536729590127535No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23610.15328342046012294No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22710.14744034217066465No Hit
GTCATACTCTTTCAAATTTGGAGGAGGGACCACACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA22340.14503818776277622No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG22120.1436098797364642No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21830.1417271100654165No Hit
GAGTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAGC21780.14140249460489104No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21700.1408831098680503No Hit
GTAATAATCAGCTTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG21150.13731233980227023No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20850.13536464703911746No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC20700.13439080065754108No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20360.13218341552596796No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20230.13133941532860174No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCACCAATGATATT19950.12952156874965914No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19560.12698956815756055No Hit
CTCCTGCACTGGAACCACCAATGATATTGATGGTTATTACTTAGTCTCCTGGTACCAACAATACCCAGGCAAAGC19460.12634033723650964No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC19280.12517172157861797No Hit
GTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC19130.12419787519704158No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19100.12400310592072632No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18690.12134125914441751No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT18510.12017264348652586No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18490.12004279730231568No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18410.11952341256547493No Hit
GTCAGTCACCATCTCCTGCACTGGAACCACCAATGATATTGATGGTTATTACTTAGTCTCCTGGTACCAACAATA18260.11854956618389854No Hit
GGACCACACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17880.11608248868390506No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17870.11601756559179995No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16960.11010956421023654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16800.10907079473655508No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16800.10907079473655508No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16800.10907079473655508No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16220.10530525539445973No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16210.10524033230235462No Hit
ACAATACCCAGGCAAAGCCCCCAAACTCATAATTTTTGATGTCAACAAGCGGCCCTCAGGGGTCCCTTATCGCTT15990.1038120242760426No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15740.10218894697341528No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15690.10186433151288983No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15420.10011140802605235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)