FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540284
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51790.336236694012273No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51270.33286069322280826No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41590.27001514006507893No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40570.2633929846703595No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTGTGGTCCCTCCTCCAAATTTGAAAGAGTATGACCCTGCATATGAGCAG35720.23190528499938973No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35060.22762036092045362No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33400.2168431276310083No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33050.21457081940733008No Hit
GTGTGGTCCCTCCTCCAAATTTGAAAGAGTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAG31550.20483235559156623No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30600.19866466184158246No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30290.19665204598632458No Hit
GATTATTACTGCTGCTCATATGCAGGGTCATACTCTTTCAAATTTGGAGGAGGGACCACACTGACCGTCCTGGGT29320.19035450605213064No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27460.17827881092058348No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27020.1754221948679594No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAC25540.16581357723640575No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25340.1645151153943039No Hit
TCTCTGGGCTCCAGGCTGAAGATGAAGCTGATTATTACTGCTGCTCATAT24990.16224280717062567No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTGTGGTCCCTC24600.15971080657852707No Hit
GTCATACTCTTTCAAATTTGGAGGAGGGACCACACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA22790.14795972690750536No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22530.14627172651277298No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22510.1461418803285628No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22270.14458372611804055No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21360.13867572473647716No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21180.13750710907858552No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21060.1367280319733244No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20810.13510495467069708No Hit
GAGTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAGC20790.1349751084864869No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG20620.13387141592070034No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC20390.1323781848022832No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20310.13185880006544248No Hit
CTCCTGCACTGGAACCACCAATGATATTGATGGTTATTACTTAGTCTCCTGGTACCAACAATACCCAGGCAAAGC19950.12952156874965914No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19210.12471725993388233No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18990.1232889519075703No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18610.12082187440757679No Hit
GTATGACCCTGCATATGAGCAGCAGTAATAATCAGCTTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGC18590.1206920282233666No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18530.12030248967073605No Hit
GTAATAATCAGCTTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18210.11822495072337309No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCACCAATGATATT18210.11822495072337309No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18110.11757571980232216No Hit
GGACCACACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18050.1171861812496916No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17790.1154981808549592No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17620.11439448828917266No Hit
GTCAGTCACCATCTCCTGCACTGGAACCACCAATGATATTGATGGTTATTACTTAGTCTCCTGGTACCAACAATA17490.11355048809180644No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17140.11127817986812821No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16720.10855140999971435No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16680.10829171763129396No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16470.10692833269708704No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16230.10537017848656481No Hit
GGGTCATACTCTTTCAAATTTGGAGGAGGGACCACACTGACCGTCCTGGG16120.1046560244734088No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15420.10011140802605235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)