FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016444
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTATGGGACCTCACCGAGGTACACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAA49140.48345014580242496No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40560.39903821558295394No Hit
GTATGGGACCTCACCGAGGTACACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCAT39240.38605176477995834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30920.30419777183986524No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30850.3035090964184943No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30540.3004592481238514No Hit
GTCCCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTC29640.2916048498490817No Hit
GTGTATTACTGTCAGCTGTATGGGACCTCACCGAGGTACACTTTTGGCCA26530.26100798469959974No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT26220.25795813640495685No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25300.24890697372408122No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25090.2468409474599683No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22460.22096642805703018No Hit
CTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAGCCTGGTACCAGCACAAAAGTGGCCAGGCTCCCAGGCTCCTCAT22290.21929393060512925No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21650.21299746960973748No Hit
CATCTATGGTGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21620.21270232300057848No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20830.20493012895939178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20610.20276572049222585No Hit
GTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAGCCTGGTACCAGCA20360.2003061654159009No Hit
GTCTGGGACAGACTTCACTCTCACCATCGACAGACTGGAGCCTGAAGATT20010.19686278830904602No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18990.1868278035976404No Hit
GTCTCCAGGCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCT18320.18023619599308965No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18270.17974428497782466No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17840.1755138502465458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17730.17443164601296282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17560.1727591485610619No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17490.17207047313969093No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTACT17120.16843033162673007No Hit
CTCCAGGCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAG17050.16774165620535908No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16810.16538048333208716No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16380.1611500486008083No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC15820.1556406452298405No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCA15790.1553454986206815No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15550.1529843257474096No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCACTTTTGTGCTGGTACCAGGCTAACTTGCCACTCTGACTGGCCCTG14990.14747492237644177No Hit
GGCCAAAAGTGTACCTCGGTGAGGTCCCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT14480.14245743002073896No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13700.13478361818260523No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCTCGGTGAGGTCCCATACAG13680.13458685377649926No Hit
GACTTCACTCTCACCATCGACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTATGGGACCTCA13620.13399656055818127No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT13620.13399656055818127No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13130.12917583260858442No Hit
GCACAAAAGTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTT13090.12878230379637245No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13080.12868392159331946No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12940.1273065707505775No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12750.12543730889257057No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12670.12465025126814659No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12530.12327290042540465No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12430.12228907839487467No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12030.11835379027275482No Hit
GTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG11800.11609099960253591No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11770.11579585299337691No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11480.11294276910484001No Hit
CTTTTGTGCTGGTACCAGGCTAACTTGCCACTCTGACTGGCCCTGCAGGA11480.11294276910484001No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11440.11254924029262803No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11400.11215571148041603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11270.11087674284072709No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11160.10979453860714412No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10750.10576086828197127No Hit
TCTATGGTGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT10710.10536733946975928No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAGCCTGGTAC10520.10349807761175234No Hit
CAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGA10520.10349807761175234No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10470.10300616659648736No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10420.10251425558122239No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10370.10202234456595739No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA350.003677250161.66000470
CGGACAA400.00623851853.95250370
GGACGGT450.0099376247.9577870
TGTCGTA1500.043.4499838
CTTATAC8500.043.3278731
TAACGGC405.2845746E-542.72459436
GGTATCA8700.042.72021
TATACAC8700.042.3297463
TCGTATG8250.041.74786840
GCCGTCT7800.041.55951346
CCGTCTT7900.041.12320747
ACGCGTA8400.041.10692227
GACGCGT8400.041.08666626
ATGCCGT8300.040.98924344
CGAGACG8750.040.95366723
TTATACA9000.040.9187552
CGCGTAG8550.040.7977828
CACGAGA8550.040.71143721
AATCTCG6400.040.58836436
CGTATGC8450.040.4231241
Produced by FastQC (version 0.11.5)