FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016444
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63580.6255140470109519No Hit
GTATTACTGTCAGCTGTATGGGACCTCACCGAGGTACACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAA45390.44655681965755123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39280.38644529359217034No Hit
GTATGGGACCTCACCGAGGTACACTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCAT36820.36224327164113324No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29220.28747279732085584No Hit
GTCCCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTC28050.27596207956365526No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27430.2698623829743695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25380.24969403134850518No Hit
GTGTATTACTGTCAGCTGTATGGGACCTCACCGAGGTACACTTTTGGCCA25070.2466441830538623No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24620.24221698391647747No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22620.22254054330587814No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21880.21526026027995643No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21650.21299746960973748No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTCGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21170.20827512386319366No Hit
GTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAGCCTGGTACCAGCA20680.20345439591359682No Hit
CTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAGCCTGGTACCAGCACAAAAGTGGCCAGGCTCCCAGGCTCCTCAT20420.2008964586342189No Hit
GTCTGGGACAGACTTCACTCTCACCATCGACAGACTGGAGCCTGAAGATT19690.19371455781135016No Hit
CATCTATGGTGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18390.18092487141446062No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17910.17620252566791678No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17030.16754489179925308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16550.16282254605270924No Hit
CTCCAGGCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGGCAAGTTAG16400.1613468130069143No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTACT16250.15987107996111935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16120.1585921113214304No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16010.15750990708784746No Hit
GTCTCCAGGCACCCTGTCTTTGTCTGCAGGGGAAAGAGCCACCCTCTCCT15660.15406652998099257No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15500.15249241473214462No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15270.1502296240619257No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15070.14826198000086577No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14980.1473765401733888No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14810.14570404272148785No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGGTCAAACGAACTGTGGCTGCACCA14620.14383478086348092No Hit
GTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACCTCGGTGAGGTCCCATACAG14550.14314610544210996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCAGCTTGGTCC13690.13468523597955226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13510.1329143563245983No Hit
GGCCAAAAGTGTACCTCGGTGAGGTCCCATACAGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT13470.1325208275123863No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13350.13134024107575035No Hit
GACTTCACTCTCACCATCGACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTATGGGACCTCA13290.13074994785743238No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13090.12878230379637245No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCACTTTTGTGCTGGTACCAGGCTAACTTGCCACTCTGACTGGCCCTG12940.1273065707505775No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12730.12524054448646457No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12700.12494539787730559No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12100.1190424656941258No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12060.11864893688191382No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11930.11736996824222486No Hit
GCACAAAAGTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGTAGGGCCACTGGCATCCCAGACAGGTT11710.11520555977505895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11610.11422173774452897No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11500.11313953351094601No Hit
GTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG11310.11127027165293907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11260.11077836063767409No Hit
CTTTTGTGCTGGTACCAGGCTAACTTGCCACTCTGACTGGCCCTGCAGGA10980.10802365895219018No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10910.10733498353081922No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10880.10703983692166022No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10660.1048754284544943No Hit
ATATATTACTGTGCGAGAGACCGCTCCGGGGGATGGGTAGAGTTACCCTC10480.10310454879954035No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10300.10133366914458641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.040.2992171
TAACTCG350.001213103140.28155551
GTATCAA24400.038.8734741
TCAACGC28750.032.7295234
ATCAACG29000.032.6847953
CAACGCA29200.032.4529385
AACGCAG29500.032.008596
ACGCAGA30900.030.6675017
TTAACGG450.00487879730.33006935
CGCAGAG31450.030.1311868
GAGTAAG450.0051681529.97482712
AGAGTAC31550.029.4939811
GCAGAGT32200.029.2184739
GTCACCG7750.028.54893970
TATCAAC34200.028.4167822
TTAGAGA953.906007E-728.4013964
TGTTAGA10250.028.0615270
GATTCAC1603.6379788E-1227.4258561
AATGGTA3707.2759576E-1225.91266470
CAGAGTA37250.025.34534510
Produced by FastQC (version 0.11.5)