FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528611
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGA20240.38289025389180326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14710.2782764641674123No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12980.2455491845610477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11960.22625333184515647No Hit
GTGTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA9790.1852023510672309No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA9630.18217555064120877No Hit
CTCCTGTAGGGCCAGACAGAGTGTTAGTAGCAACTTCTTAGCCTGGTTTC9570.18104050048145046No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9560.18085132545482407No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9310.17612194978916446No Hit
GTAATATAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATAGA8180.1547451717803829No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7830.14812404584845945No Hit
GTCTGAGACCTGAGGACACCGCCTTATATTACTGTGCAAACCTACCTACC7610.14396219526267898No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7590.14358384520942621No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7420.14036786975677767No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGACAGAGTGTTAGTAG7360.13923281959701936No Hit
GTGTTAGTAGCAACTTCTTAGCCTGGTTTCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT7240.13696271927750273No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7170.13563849409111806No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7060.1335575687982278No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCA7050.13336839377160142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7010.1326116936650959No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6930.13109829345208482No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6900.13053076837220565No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6850.1295848932390737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6710.12693644286630432No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6690.12655809281305158No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6580.12447716752016133No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6350.12012614190775447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6220.11766686656161146No Hit
CTCTTATTACTTGGGATGGGGGTAGTACATTCTATGCAGACTCTGTGAAG6220.11766686656161146No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT6130.115964291321974No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6080.11501841618884207No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTGGT5940.11236996581607268No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT5910.11180244073619354No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGAAACCAGGCTAAGAAGTTGCTACTAACACT5860.1108565656030616No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5830.11028904052318245No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5760.10896481533679775No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5410.10234368940487427No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5380.10177616432499512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5360.10139781427174237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5350.10120863924511599No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5310.10045193913861042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAGC150.002406478468.6235332
TTTCGAT757.310864E-752.65723470
GCCGTCT2350.048.7757246
CGTATGC2350.048.46801441
TCGTATG2300.047.98890340
TAACGGC652.2009772E-1047.63987736
CGTACTA2400.047.0847229
ACCGTAC2450.046.06701727
GACCGTA2450.046.0539326
CGGTACC704.1836756E-1044.606845
AGACCGT2550.044.24370225
TTAACGG704.5838533E-1044.19912335
GTATGCC2600.043.8537442
ATCTCGT2200.043.8068837
GTACTAG2600.043.48343730
CGTCTTC2650.043.34984648
CCGTCTT2650.043.2872547
CTAGATC2250.042.727533
TACTAGA2650.042.70756531
ATGCCGT2700.042.33078844
Produced by FastQC (version 0.11.5)