FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528611
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGA18680.35337894973808714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18570.3512980244451969No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13670.25860226139826825No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11560.21868633078010105No Hit
CTCCTGTAGGGCCAGACAGAGTGTTAGTAGCAACTTCTTAGCCTGGTTTC8770.16590649835133964No Hit
GTGTATTACTGTCAGCAATATGGTAGTTCACGTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8590.16250134787206472No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8350.15796114723303148No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8160.15436682172713018No Hit
GTCTGAGACCTGAGGACACCGCCTTATATTACTGTGCAAACCTACCTACC8100.15323177156737186No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8090.15304259654074548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8000.151340021301108No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7890.14925909600821777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7370.13942199462364574No Hit
GTGTTAGTAGCAACTTCTTAGCCTGGTTTCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT6920.1309091184254584No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6880.13015241831895288No Hit
GTAATATAAGGCGGTGTCCTCAGGTCTCAGACTGTTCATTTGCAGATAGA6840.12939571821244733No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGACAGAGTGTTAGTAG6750.12769314297280987No Hit
CTCTTATTACTTGGGATGGGGGTAGTACATTCTATGCAGACTCTGTGAAG6680.1263689177864252No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6450.12201789217401833No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCA6330.11974779185450171No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6170.11672099142847954No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6040.11426171608233654No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5850.11066739057643522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5830.11028904052318245No Hit
GTCCCAGACCCACTGCCACTGAATCTGTCTGGGATGCCAGTGGCCCTGGT5710.10801894020366584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5690.10764059015041305No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5630.10650553999065476No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT5610.10612718993740199No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5590.10574883988414921No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5340.10101946421848958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5320.10064111416523683No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC5320.10064111416523683No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5300.10026276411198405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGC200.007559160251.39607631
GAGTAAG352.4243773E-548.7446751
GGTATCA6850.046.8233531
GACAGGT1050.042.2453841
TAACGTC350.001414998939.03640426
GTATCAA17350.037.56291
CAGTTCG6150.036.46255570
AACTGTG8100.036.07378470
TATCAAC19850.032.316372
ATCAACG20050.031.8087673
ATTAGGT450.004123320331.38828357
TAACGGC553.3115243E-431.27107236
TCAACGC20300.031.0721994
GCATAAG553.4722633E-431.0193391
TGACCGA450.00489939730.30132310
CAACGCA20900.030.1773195
GTACCCT703.662143E-529.7855947
GTGGTAT2750.029.7785641
AACGCAG21300.029.7706646
CGGTACC703.6960315E-529.74542445
Produced by FastQC (version 0.11.5)