FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389418
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT65220.46940517540437793No Hit
GTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC55520.39959177151872227No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54770.3941938279193159No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48750.35086633396141403No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43880.31581568685593536No Hit
ATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA43760.31495201588003036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40360.29048133822938815No Hit
GTGTAGTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATT40070.28839413337095104No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37790.27198438482875564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37650.27097676869019977No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA36420.2621241411871733No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGCTTTGTGCTGATACCAATT35310.2541351846600519No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCGTTAGTGG32590.23455864253953815No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31970.2300963424973622No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31240.22484234406060666No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30910.22246724887686786No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCGTTAGTGG30820.2218194956449391No Hit
GAATTCACTCTCACCATCAGCAGCCTGCTACCTGAAGATTTTGCAACCTACTATTGTCAACAGACTTATACTACA30280.21793297625336655No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30120.21678141495215983No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28730.20677722614792668No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28570.20562566484671999No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28310.2037543777322591No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28230.20317859708165578No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28030.2017391454551474No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27290.19641317443706646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26810.19295849053344638No Hit
TCCCAGGTGTAGTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCA26300.18928788888585005No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC26170.1883522453286196No Hit
CTACTATTGTCAACAGACTTATACTACACCTGGGACGTTCGGCCAAGGGA25920.18655293079548413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25890.1863370130515079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25790.18561728723825371No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA25070.1804352613828236No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24340.17518126294606806No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24260.17460548229546471No Hit
CACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC23070.16604074511773995No Hit
GCACAAAGCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT22700.16337775960869946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22610.1627300063767707No Hit
GCTTTGTGCTGATACCAATTTAAATAGCCACTAACGGTCTGACTTGCCCGACAAGTGATGGTGACT21930.15783587084664227No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21790.15682825470808642No Hit
CAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA21220.1527258175725376No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20500.14754379171710746No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT20300.14610434009059908No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20020.14408910781348738No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19910.14329740941890778No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19500.1403465335845656No Hit
GGTTAGGGGCTTTCCCTGCTTTGTGCTGATACCAATTTAAATAGCCACTA19440.1399146980966131No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGA19320.13905102712070808No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19270.13869116421408098No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19090.13739565775022347No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18660.13430083675323048No Hit
AAGCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCAGTTTGCAA18190.13091812543093584No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18140.13055826252430874No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17750.12775133185261742No Hit
ATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA17020.1224973334158619No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGCTTTGTG16780.12076999146405185No Hit
ACCTACTATTGTCAACAGACTTATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16540.11904264951224182No Hit
TCCCTGCTTTGTGCTGATACCAATTTAAATAGCCACTAACGGTCTGACTTGCCCGACAAGTGATGGTGACT16410.11810700595501138No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15620.11242117203030333No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15410.11090974782246955No Hit
CACCATCAGCAGCCTGCTACCTGAAGATTTTGCAACCTACTATTGTCAACAGACTTATACTACACCTGGGACGTT15400.11083777524114413No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15370.11062185749716788No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15320.11026199459054078No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15320.11026199459054078No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15240.10968621393993745No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15230.10961424135861203No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15160.1091104332893341No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCTACCTGAAGATTTTG15130.10889451554535784No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14840.10680731068692072No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14720.1059436397110157No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCTTCAGGGAGA14570.10486405099113442No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14500.10436024292185649No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG14500.10436024292185649No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14480.10421629775920564No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA14430.10385643485257856No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14340.1032086816206498No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14230.1024169832260702No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14200.10220106548209396No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14070.10126542192486351No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCTACCTGAAGATTTTGCAACCTA14050.10112147676221267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)