FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389418
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT62230.4478853735880779No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52030.3744733406361513No Hit
GTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC50780.36547676797047396No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41910.30163708833482794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40960.29479969310891324No Hit
ATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA39850.2868107365817918No Hit
GTGTAGTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATT39360.2832840800968463No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37390.26910548157573894No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36250.2609006073046412No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34960.25161614431366225No Hit
GATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA32550.2342707522142365No Hit
CCATAGATCAGGAGGTTAGGGGCTTTCCCTGCTTTGTGCTGATACCAATT31110.22390670050337624No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30150.21699733269613608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29810.21455026493107185No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCGTTAGTGG29650.21339870362986516No Hit
GAATTCACTCTCACCATCAGCAGCCTGCTACCTGAAGATTTTGCAACCTACTATTGTCAACAGACTTATACTACA29330.2110955810274518No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGACCGTTAGTGG28450.204761993870815No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27880.20065955673526614No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27640.1989322147834561No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27380.19706092766899522No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27040.19461385990393099No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26570.19123114858163634No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC26350.18964775179247714No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25860.18612109530753165No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24770.17827608394306105No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24530.176548741991251No Hit
TCCCAGGTGTAGTATAAGTCTGTTGACAATAGTAGGTTGCAAAATCTTCA24360.1753252081087189No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24170.17395772906353596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24150.17381378390088512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23980.17259025001835301No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23090.1661846902803908No Hit
CTACTATTGTCAACAGACTTATACTACACCTGGGACGTTCGGCCAAGGGA23030.16575285479243826No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22800.16409748542195365No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA22440.16150647249423858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22350.16085871926230982No Hit
CACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC20620.14840746269301247No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20370.14660814815987702No Hit
GCACAAAGCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT20300.14610434009059908No Hit
GCTTTGTGCTGATACCAATTTAAATAGCCACTAACGGTCTGACTTGCCCGACAAGTGATGGTGACT20230.14560053202132117No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19920.1433693820002332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19680.14164204004842315No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCAAGTCAGA19670.14157006746709772No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19620.14121020456047065No Hit
GGTTAGGGGCTTTCCCTGCTTTGTGCTGATACCAATTTAAATAGCCACTA19430.1398427255152877No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18730.13480464482250842No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC18730.13480464482250842No Hit
CAATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA18640.13415689159057964No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18360.13214165931346794No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18330.1319257415694917No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT17950.1291907834791258No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17440.12552018183152946No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT17290.12444059311164819No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17080.1229291689038144No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16640.119762375325496No Hit
AAGCAGGGAAAGCCCCTAACCTCCTGATCTATGGTGCATCCAGTTTGCAA16050.11551599302729633No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15760.11342878816885918No Hit
TCCCTGCTTTGTGCTGATACCAATTTAAATAGCCACTAACGGTCTGACTTGCCCGACAAGTGATGGTGACT15710.11306892526223211No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15560.11198933654235084No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT15550.1119173639610254No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15520.11170144621704915No Hit
ATAGTAGGTTGCAAAATCTTCAGGTAGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA15260.10983015910258828No Hit
ACCTACTATTGTCAACAGACTTATACTACACCTGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14860.10695125584957155No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14630.10529588647908693No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14610.10515194131643608No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTCCCTGCTTTGTG14480.10421629775920564No Hit
CACCATCAGCAGCCTGCTACCTGAAGATTTTGCAACCTACTATTGTCAACAGACTTATACTACACCTGGGACGTT14360.10335262678330064No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14320.10306473645799896No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14310.10299276387667354No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14160.10191317515679227No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13960.1004737235302839No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGTTCACCTTCAGGGAGA13950.10040175094895849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT200.00651172953.3762556
GTGTACG200.007502507451.498439
GTGGTAT4400.046.898941
GTTACGG350.001451998538.83635731
GTATCAA30050.035.911381
GACAGGT3050.034.3833161
AACTAAT7050.033.31283670
CGCAACA450.00346791832.52162664
ATACGTA1307.2759576E-1231.18832211
TATCAAC34250.031.1024842
ATCAACG34750.030.6395113
TCAACGC35050.030.466024
AACGCAG35550.030.130416
CAACGCA35600.029.9931775
TACGTAC1401.8189894E-1128.96267112
AATACGT1401.8189894E-1128.96058710
ACGCAGA37300.028.7167857
CGCAGAG38050.028.0619478
GAATACG1452.910383E-1127.9629529
TCATCGA500.007515869627.76330845
Produced by FastQC (version 0.11.5)