FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152457
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG41560.36062083010472407No Hit
GATTATTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTTGGGTGTTCGG39450.34231212097284325No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29950.2598795443127162No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26880.23324080638149622No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24090.20903166018341682No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23670.20538727258370593No Hit
GCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCACG22880.1985323530509164No Hit
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTCCATTTGCAGATACA20800.18048395731901493No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20380.17683956971930406No Hit
GTCTATTACTGTGCGAGGAGTGGTATCAGGCCATTTGACTACTGGGGCCA19700.1709391326531055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19460.1688566254532707No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18920.16417098425364243No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18240.15827054718744385No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18210.15801023378746454No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17880.15514678638769167No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGCCACCTTGGTCCCTC17680.15341136372116268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17460.1515023987879808No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17280.1499405183881047No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16770.1455151905884558No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGGTACCAGTTTACAGTCTTACTTCCGATGTTTGAG16580.14386653905525326No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16480.14299882772198874No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16470.1429120565886623No Hit
GACCTAGGACGGCCACCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG16360.14195757412207136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16040.13918089785562499No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAT15930.13822641538903405No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15040.13050378452298003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14950.12972284432304199No Hit
CCCCTGAGGGCCGCTGATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGGTACCAGTTTA14880.12911544638975683No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14700.12755356598988074No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14510.1259049144566782No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14250.12364886499019052No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14090.12226052685696734No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13980.1213060443903764No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13930.12087218872374414No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGATAATGCCAAGAATTCACTGTATCTG13820.1199177062571532No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13600.11800874132397131No Hit
CATCTATAGTGATAATCAGCGGCCCTCAGGGGTCCCTGACCGCTTCTCTGGCTCCAGGTCTGGCACCTCAGCCTC13320.11557914959083072No Hit
GATTATCACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCCGCTGG13240.11488498052421912No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12980.11262893105773145No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12960.11245538879107854No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCGTGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC12690.11011256819126441No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAAGCGGTCAGGGACC12500.10846391665806185No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12420.10776974759145028No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12410.10768297645812383No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12390.10750943419147092No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12350.10716234965816512No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12200.10586078265826838No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12170.10560046925828903No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12070.10473275792502454No Hit
GTCTTACTTCCGATGTTTGAGCTGCTTCCAGAACAAGAGATGATGACCCT12030.10438567339171874No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAG11970.10386504659176005No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11780.10221639505855751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)