FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004686653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599933
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA27940.17463231272809548No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25240.15775660605787867No Hit
GTGTAACTCTGTTGACAGTAATAAACTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTC24970.156069035390857No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23880.14925625010547316No Hit
CCATAGATCAGGACCTGAGGGGCTTTCCCTGGCTTCTGCTCATACCAATT23620.14763118205574857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22590.14119341247414735No Hit
GTAATAAACTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCG20590.1286928890147275No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20330.1270678209650029No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20130.1258177686190609No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19320.12075505661799588No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18660.11662988387638733No Hit
GAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT18240.11400477394990914No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18230.11394227133261206No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18120.11325474254234395No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17430.10894206194884411No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17410.1088170567142499No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTTCTGGAGCTGGATCCGGCAGCC17350.10844204101046731No Hit
GTCTCCATCCTCCCTGTCTGCATCTTTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAA17120.10700448081263404No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16930.10581693108398915No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16810.10506689967642395No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16630.10394185256507617No Hit
GTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTGGTGGGACCCCA16260.1016292557250835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)