FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599933
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43600.272511411415353No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA25270.15794411390976998No Hit
GTGTAACTCTGTTGACAGTAATAAACTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTC23840.14900623963628476No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20930.13081797800282888No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20180.12613028170554644No Hit
CCATAGATCAGGACCTGAGGGGCTTTCCCTGGCTTCTGCTCATACCAATT20110.12569276338446672No Hit
GTAATAAACTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCG20040.12525524506338703No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19440.12150508802556106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19040.1190049833336771No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18380.11487981059206853No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18140.11337974777693816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17900.11187968496180778No Hit
GAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16890.10556692061480075No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTTCTGGAGCTGGATCCGGCAGCC16650.10406685779967037No Hit
GTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTGGTGGGACCCCA16490.10306681592291678No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16390.10244178974994576No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16050.1003167007618444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)