FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487012
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG100700.6771969560433944No Hit
GATTATTACTGTGCAGCATGGGATGACAGGCTGAATGCTGTGATATTCGG97960.6587707429395324No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70180.47195315168942814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61670.41472429274276196No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAGCATTCAGCCTGTCATCCCATG61600.41425355007222536No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG57120.3841260191578817No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT52520.3531915008083324No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48370.32528318534080425No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCGGGGACCC47420.318894534812093No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC46730.3142543570596606No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAGCATTCAGC46260.3110936562717719No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42700.2871530290273381No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42540.2860770457803972No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40210.27040803974682115No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT40160.2700717949821521No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39810.26771808162946903No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC39460.26536436827678594No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCG39330.2644901318886465No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36580.24599666983185073No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35150.23638006956231689No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG35080.2359093268917803No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34500.23200888762161972No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG34270.23046216170414224No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34110.2293861784572014No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT33420.22474600070476902No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32770.22037481876407183No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC32030.2153983962469704No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31780.21371717242362537No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31520.2119686996473465No Hit
CATTACTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGG30650.20611804074210566No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA30630.20598354283623807No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29490.19831716220178452No Hit
CCCCTGAGGGCCGCTCATTACTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGGTACCAATCTA29170.19616519570790283No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28720.1931389928258817No Hit
GCATGGGATGACAGGCTGAATGCTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG28340.1905835326143972No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGGCTGAATGCTGTGATATTCGGCGGAGGGAC28340.1905835326143972No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT27930.18782632554411127No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG27910.18769182763824366No Hit
GTGCTGACTCAGCCACCCTCAGCGTCGGGGACCCCCGGGCAGAGGGTCAC27750.18661584439130283No Hit
GTAGTAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG27410.18432937999155352No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27130.18244640930940706No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC26910.18096693234486338No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26650.1792184595685845No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26380.17740273783937183No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26320.176999244121769No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25560.1718883236988No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25240.1697363572049183No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC24770.16657565641702957No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAG24450.16442368992314788No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACAGCATTCAGCC23810.1601197569353845No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23730.1595817653119141No Hit
GAAGTAATACTGTAGATTGGTACCAACAACTCCCAGGAACGGCCCCCAAA23510.15810228834737045No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22920.15413460012427607No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC22790.15326036373613663No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG22290.1498979160894465No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTCATTA22200.14929267551304226No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22160.14902367970130706No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC22130.14882193284250564No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC21960.147678700642631No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC21640.1455267341487493No Hit
CTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGGTACCA21580.1451232404311465No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21200.142567780219662No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA20890.1404830626787141No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20670.13900358571417043No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG20650.13886908780830284No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20560.1382638472318986No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20460.1375913577025606No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20400.13718786398495775No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20300.13651537445561973No Hit
GTGCAGCATGGGATGACAGGCTGAATGCTGTGATATTCGGCGGAGGGACC20240.13611188073801692No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20060.13490139958520847No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20020.13463240377347324No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT20010.13456515482053946No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19950.13416166110293665No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19650.13214419251492254No Hit
TTACTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGTTGGTA19540.13140445403265072No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC19280.12965598125637184No Hit
GCATTCAGCCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC19240.12938698544463664No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18840.12669702732728452No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGTTGGTACCAATCTACAGTATTACTT18770.1262262846567479No Hit
GCTTGGTCCCTCCGCCGAATATCACAGCATTCAGCCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT18700.12575554198621128No Hit
GTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG18680.12562104408034366No Hit
CCTGAGGGCCGCTCATTACTACTATAGATGAGGAGTTTGGGGGCCGTTCC18470.12420881606873381No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAGATTGGTACCAACAACTCCCAGGAACGGCCCC17770.11950138936336761No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17750.11936689145750001No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17750.11936689145750001No Hit
GGATGACAGGCTGAATGCTGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17610.11842540611642677No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17550.11802191239882395No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17500.11768566763415494No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17470.11748392077535352No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG17360.1167441822930817No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA17180.11553370114027325No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17070.11479396265800142No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16840.11324723674052395No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16600.11163326187011269No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG16420.11042278071730423No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC16270.10941404642329719No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG16110.10833806317635634No Hit
TTACTGTGCAGCATGGGATGACAGGCTGAATGCTGTGATATTCGGCGGAG15990.10753107574115071No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15940.10719483097648169No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTC15810.10632059458834225No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15750.10591710087073944No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGACTATTCATTTGTAGATATA15620.1050428644826No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15560.10463937076499719No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC15250.1025546532240493No Hit
GGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGGCTGAATGCTGTGATATTCGGCGGAGGGACCAAG15200.10221840845938028No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG15180.10208391055351268No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15180.10208391055351268No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15090.10147866997710846No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15020.10100792730657183No Hit
TAGTAGTAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14970.10067168254190283No Hit
CCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATC14960.10060443358896902No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14910.1002681888243No Hit
CTATAGTAGTAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14880.10006644196549859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGC200.00656001753.2768864
GTGGTAT3050.049.4346661
TGGTATC3350.047.0534822
GGTATCA12050.043.5094071
AATCGCG503.9080805E-641.2998436
GTATCAA30950.041.1870841
CTCTTAT1450.040.175351
GCCAGAG42250.036.76800570
TCTTATA1700.036.282932
CCGGCAT400.002272275735.45118363
TATCAAC36300.035.4000132
TATACAT400.00268422534.2672125
GTACGGA400.00268422534.2672123
TATACAC3600.034.267213
ATCAACG38350.033.4183463
TCAACGC38650.033.158964
CAACGCA39150.032.7354745
TCTGGAG8950.032.69024770
AACGCAG39750.032.4137656
AATGGTA2157.4578566E-1032.4006370
Produced by FastQC (version 0.11.5)