FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762688
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20360.26695057480909623No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCTAATATCAC17000.22289586305278175No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16050.21043991776453805No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14710.19287047914743644No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC13890.18211903163547874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12740.16704078207602585No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11910.15615821935050767No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA11590.15196253251657296No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCTAATATCACAGATGCACTC11570.15170030208945204No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA10660.13976881765545018No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG10410.13649093731643872No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10380.13609759167575733No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10280.13478643954015274No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10100.13242636569606445No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10090.13229525048250398No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9710.1273128723672065No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9520.12482168330955777No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9410.12337941596039272No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9320.12219937903834859No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9300.12193714861122766No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9140.11983930519426031No Hit
GATTATTACTGCGGAACATGGGATGCCAGCCTGAGTGCATCTGTGATATTAGGCGGAGGGACCAAACTGACCGTC9100.11931484434001846No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8900.11669254006880926No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8840.11590584878744652No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT8760.11485692707896282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8730.11446358143828145No Hit
CAGTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATA8590.112627968448435No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8540.1119723923806327No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8400.11013677939078627No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8350.10948120332298399No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8350.10948120332298399No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8320.1090878576823026No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8230.10790782076025844No Hit
GTATTACTGTGCAAAAGATCGGGATGATAGTCGTGGTTATTCCATTGACTACTGGGGCCAGGGAACCCTGGTCAT8130.10659666862465386No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8050.1055477469161702No Hit
GTTTGGTCCCTCCGCCTAATATCACAGATGCACTCAGGCTGGCATCCCATGTTCCGCAGTAATAATCGGCCTCGT7930.10397436435344465No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7860.10305655785852144No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7850.10292544264496098No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG7740.10148317529579591No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7700.10095871444155409No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG7670.1005653688008727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT350.004649198658.11206470
AGAACGA200.00690654352.585453
GCGTATG200.00735803551.7500540
TGGTATC2600.043.3750762
CTACGCT2150.042.95881728
GTGGTAT2400.042.720681
ACTACGC2250.042.56151227
GGTATCA8250.041.4261171
TACGCTG2300.040.167729
ATCTCGT955.456968E-1239.8392937
CGTGAAA458.8084984E-539.1761257
GTATCAA20350.038.7951
GACTACG2500.038.30284526
ACGAGAC2500.038.27271322
CGAGACT2550.037.52226623
CGTATGC655.3804433E-737.21299741
ACGCTGC2550.036.24872630
TCTCCGA2700.035.437711
GACAGGT1950.035.0528681
CACGAGA2450.034.86945321
Produced by FastQC (version 0.11.5)