FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253574
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCACTATGGTACCTCATCGGGGACTTTCGGCGGAGGGA58670.4680218319780085No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39270.3132643146714913No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA38360.3060050703029897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30110.2401932394896512No Hit
GTGTATTACTGTCACCACTATGGTACCTCATCGGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA29920.23867757308304094No Hit
GTACCATAGTGGTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC29610.23620464368278218No Hit
CTATGGTACCTCATCGGGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT29500.235327152605271No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28510.2274297329076704No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26510.21147534968019438No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25170.20078591291778544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24680.19687708902705386No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23460.1871449152582935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23380.18650673992919445No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21880.17454095250858745No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20920.16688284855939897No Hit
CATCTATGGTGCTTCCAGCAGGGCCGCTGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19690.15707090287450123No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19190.1530823070676322No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19180.15300253515149484No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18720.14933302700917536No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTCCTTAGCCTGGTCCC18530.14781736060256515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18420.14693986952505397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17620.14055811623406356No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCAGCGGCCCTGCT17580.14023902856951403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17580.14023902856951403No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17110.1364897485110572No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17110.1364897485110572No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17010.13569202934968339No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16620.13258092462032559No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16220.12939004797483036No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCA16030.12787438156822015No Hit
GTAATACATGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA16020.12779460965208275No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGGGACCAGGCTAAGGAGCTGCTGCCAACACTCTGA15920.12699689049070895No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15810.12611939941319777No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15740.12556099600023612No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15590.12436441725817543No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG15510.12372624192907639No Hit
GGTTTGTGCTGGGACCAGGCTAAGGAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTCC15310.12213080360632879No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15190.12117354061268024No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14380.11471201540555244No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA14210.11335589283121697No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC14020.11184022642460677No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14010.11176045450846939No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13980.11152113876005723No Hit
GGCTAAGGAGCTGCTGCCAACACTCTGACTGGCCCTGCAGGAGAGGGTGG13760.1097661566050349No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13350.1064955080434023No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC13200.1052989293013416No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13170.10505961355292946No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13000.10370349097859399No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCTTCCAGCAGGGCCGCTGACATCCCAGACAGGTT12910.1029855437333576No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12740.10162942115902213No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12670.10107101774606045No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12550.1001137547524119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)