FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320773
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43690.3307911351912857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40130.3038372225961615No Hit
GTAATAAGTGGCCGTGTCCTCGGCTCTCAGGCCATTCATTTGCAGATACA39220.2969473179721269No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG33700.25515361080215904No Hit
CTACAAACCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGGGATCAAACG33630.25462361813877177No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC30100.22789684525652779No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29830.22585258784060544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29770.22539830841484493No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29620.22426260985044366No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28570.21631271989963455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28450.2154041610481135No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27710.20980138146373373No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT26270.19889867524548124No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG25830.1955672927899041No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25080.1898887999678976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24310.18405888067063758No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23780.18004607907641965No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23630.17891038051201835No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23550.1783046746110043No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC23530.1781532481357508No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22760.1723233288384908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22230.16831052724427287No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21800.16505485802632247No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT21750.1646762918381887No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21170.160284924055837No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21120.1599063578677032No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20720.15687782836263311No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19920.1508207693524928No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19790.14983649726334503No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA19180.14521798976811306No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCCCCACCTTGGTCC18620.14097804846101486No Hit
GTGCAGCCACAGTTCGTTTGATCCCCACCTTGGTCCCTCCGCCGAAAGTG18580.14067519551050786No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACCCCCCTCACTTTC18480.13991806313424032No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18320.13870665133221227No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18100.13704096010442368No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG17870.13529955563900836No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17570.13302815851020577No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT17160.12992391576750886No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17000.1287125039654808No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16940.1282582245397203No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16920.1281067980644668No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16750.12681967302481198No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16740.12674395978718522No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC16710.12651682007430498No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT16500.12492684208414316No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16440.12447256265838262No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16410.12424542294550237No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16310.12348829056923483No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15880.12023262135128444No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15550.11773408450960157No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15460.1170526653709608No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15450.11697695213333403No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15390.11652267270757352No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15100.11432698881639768No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14870.11258558435098234No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAGAATCAGCAGAG14650.11091989312319378No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGGGATCAAACGAA14490.10970848132116572No Hit
CTTATTACTGTGCGAGCCCCCAGTTGGAGAGAACCTTTTACTATGAGAGT14220.10766422390524337No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14150.10713423124185609No Hit
CAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA14080.10660423857846882No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14080.10660423857846882No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13930.10546854001406752No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13640.10327285612289168No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13590.1028942899347579No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13540.10251572374662414No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13510.10228858403374388No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13400.10145573841984959No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC13360.10115288546934256No Hit
GGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT13330.1009257457564623No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13290.10062289280595531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT150.002414572868.5712318
TCTATCG350.004844935657.51456570
CTTATAC2350.045.248391
TATACAC2450.043.3998763
GGTATCA9750.042.9204751
TCTGGAG13050.042.67683870
TGGTATC2700.041.92212
ACGTAGA2400.041.4661327
CGAGACG2400.041.43943823
GACGTAG2450.040.6091126
AGACGTA2450.040.60141825
TTATACA2650.040.1244132
GTATCAA25100.039.630871
TCGTATG1400.039.499140
CGTAGAG2600.038.28513728
ACGAGAC2650.037.53005622
CACGAGA2650.037.52721421
CGTATGC1400.037.06435441
GAGACGT2700.036.84063324
GTGGTAT2900.036.66681
Produced by FastQC (version 0.11.5)