FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320773
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41230.31216567873510437No Hit
GTAATAAGTGGCCGTGTCCTCGGCTCTCAGGCCATTCATTTGCAGATACA33650.2547750446140253No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG32710.24765800027711043No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29940.22668543345449976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29710.22494402898908442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29260.2215369332958805No Hit
GTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAATCTGTGCCTGATCCACTGCCA29110.22040123473147924No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29100.22032552149385248No Hit
CTACAAACCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGGGATCAAACG28850.21843269055318365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28170.2132841903945644No Hit
GATTTTACACTGAGAATCAGCAGAGTGGAGGCTGACGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACC27940.21154278592914907No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26140.19791440315633343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25360.1920087706214467No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24600.1862545645618134No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT23660.1791375202248986No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG21840.16535771097682947No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21650.16391915946192118No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC21460.16248060794701286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21010.15907351225380895No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20700.15672640188737957No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20300.15369787238230945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19990.1513507620158801No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19940.15097219582774635No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19840.1502150634514788No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19580.14824651927318322No Hit
GGGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT19300.1461265486196341No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19280.1459751221443806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19120.14476371034235255No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18880.14294659263931045No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17910.1356024085895154No Hit
ATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACCCCCCTCACTTTC17380.13158960699529745No Hit
GTGCAGCCACAGTTCGTTTGATCCCCACCTTGGTCCCTCCGCCGAAAGTG17270.13075676138140316No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17080.12931820986649487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17050.1290910701536146No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17030.12893964367836108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16860.12765251863870627No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16860.12765251863870627No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCCCCACCTTGGTCC16400.12416970970787562No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT15950.12076261401467173No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAG15900.12038404782653794No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15860.12008119487603092No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15860.12008119487603092No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15550.11773408450960157No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15450.11697695213333403No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15420.11674981242045378No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15420.11674981242045378No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15340.11614410651943974No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA15210.11515983443029194No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG14850.11243415787572883No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14580.1103899004598065No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14580.1103899004598065No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC14310.10834564304388415No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC14190.10743708419236311No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAGAATCAGCAGAG13790.10440855468729297No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13760.10418141497441272No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13750.10410570173678595No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13600.10297000317238465No Hit
GTTTATTACTGCATGCAAGCTCTACAAACCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGGGATCAAACGAA13520.10236429727137061No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13440.1017585913703566No Hit
GGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCGTCAGCCTCCACTCTGCTGATTCTCAGTGTAAAAT13360.10115288546934256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA200.00755571551.4063325
GTGGTAT3700.047.213181
TGGTATC3800.044.1646042
TTGTCTA350.001398748639.13259518
GTATCAA23850.039.0638771
TCTGGAG12600.038.67566770
GGTATCA9300.035.7259371
TATCAAC27100.033.49182
CTGGTAG1052.3518826E-433.15057470
ATCAACG27950.032.4609643
TCAACGC28000.032.2672774
GCTTGTA1305.456968E-1231.6178761
CAACGCA28850.031.314225
TACGTAC553.4150202E-431.11169612
AACGCAG29250.031.0029876
TAAGTCA900.00442453330.94053370
TCCGAGA8100.030.08107270
CGCAGAG30950.029.5212068
AGAGTAC30950.029.40952711
GTGAGCG7500.028.77469470
Produced by FastQC (version 0.11.5)