FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478964
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGCGCCTCATATGCAGGCAGCAACAACTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT38070.25740991667139973No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33680.22772697645108333No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31890.21562390970976983No Hit
GTAATACACGGCCGTGTCGTCGACTCTCAGGTTGTTCATTTGGAGATACA31100.2102823327680728No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31050.20994425827809193No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAGTTGTTGCTGCCTGCATATGAGGCG28820.1948661360249472No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27650.1869551929593959No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG27300.18458867152953012No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27130.18343921826359533No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAGTTGTTGCTGCCTGCATATGAGGCGCAGTAATAAT24470.16545365539661547No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23900.16159960621083408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22610.1528772843693288No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22320.15091645232744003No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22180.14996984375549371No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21580.1459129498757238No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21500.1453720306917545No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21030.14219413048593477No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20960.1417208261999616No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20720.14009806864805363No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20120.13604117476828376No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT19540.13211951068450617No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18680.12630462945683602No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCGCCTCATAT18510.12515517619090122No Hit
CTCCTGCACTGGCACCAGCAGTAACGTTGGTGGTTATAACTACGTCTCCTGGTACCAACAGCACCCAAACAAAGC18420.12454664210893572No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17970.1215039716991083No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17570.1187993757792617No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17540.1185965310852732No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17060.1153510159814573No Hit
CAGTAATACACGGCCGTGTCGTCGACTCTCAGGTTGTTCATTTGGAGATA16970.11474248189949181No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16880.11413394781752631No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16820.11372825842954934No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGCACCAGCAGTAACGTT16540.11183504128565672No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16490.11149696679567589No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16460.11129412210168739No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16430.1110912774076989No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16110.10892760067182161No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15730.10635823454796738No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14810.10013766393232021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.043.8347431
AGGGGGG4550.042.495870
TATCTCG3950.041.72443436
CGTATGC5000.041.4718441
TCGTATG4650.040.81976340
ATCTCGT4050.040.71922337
GTATCAA40450.039.8623851
ATGCCGT5150.038.46214744
GACGGAC5350.037.53502726
CACGAGA5350.037.51091421
CGAGACG5450.037.45181723
TATACAC5650.037.327773
CGGACTC5300.037.2961628
ACGGACT5300.037.264627
TATGCCG5400.037.24525543
ACGAGAC5600.037.0511122
GTGGTAT4100.036.506671
CTTATAC5600.036.447411
TCTCGTA4450.036.3465938
TCCTATC4750.036.0236133
Produced by FastQC (version 0.11.5)