FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478964
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49070.3317863044671811No Hit
GATTATTACTGCGCCTCATATGCAGGCAGCAACAACTTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT36730.24834952033991362No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32350.2187341950175934No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31930.21589436930175449No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28280.1912149315331543No Hit
GTAATACACGGCCGTGTCGTCGACTCTCAGGTTGTTCATTTGGAGATACA26780.18107269683372954No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAGTTGTTGCTGCCTGCATATGAGGCGCAGTAATAAT24680.16687356825453495No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAAGTTGTTGCTGCCTGCATATGAGGCG24560.16606218947858095No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24100.16295190417075736No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23950.1619376807008149No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22680.15335058865530193No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22180.14996984375549371No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21200.14334358375186956No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21140.14293789436389256No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21030.14219413048593477No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20810.14070660273001911No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG20130.1361087896662799No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20100.13590594497229141No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19360.1309024425205752No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19000.12846830619271327No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18640.12603416986485136No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18400.12441141231294339No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17910.1210982823111313No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17840.12062497802515816No Hit
CTCCTGCACTGGCACCAGCAGTAACGTTGGTGGTTATAACTACGTCTCCTGGTACCAACAGCACCCAAACAAAGC17600.1190022204732502No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17580.11886699067725787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16940.11453963720550332No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCGCCTCATAT16840.11386348822554167No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16510.11163219659166822No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGCACCAGCAGTAACGTT16410.11095604761170658No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16360.11061797312172575No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16300.11021228373374876No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15340.10372125352611694No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15260.10318033434214761No Hit
CAGTAATACACGGCCGTGTCGTCGACTCTCAGGTTGTTCATTTGGAGATA15250.10311271944415144No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15210.1028422598521668No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15060.1018280363822243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42100.039.4871251
GGTATCA16350.038.8823621
TATCAAC47600.034.7699282
ATCAACG49650.033.2579883
GTGGTAT5750.033.109221
TCAACGC49800.032.8793454
AACGCAG50350.032.7225956
CAACGCA50450.032.5230645
CGCAGAG51900.031.4180628
AGAGTAC52250.031.33339711
CAGAGTA52650.031.03188310
GCAGAGT54000.030.3220659
GGATGGG10800.030.29787370
AATGGTA4600.027.83509670
AAAACCG2304.1914973E-827.83509670
ACGCAGA62350.026.5337127
TGGTATC7100.026.3260772
ATGTGAT4300.024.81423470
TCGCTAT700.001459160624.26648313
GAGTACG49600.024.24527412
Produced by FastQC (version 0.11.5)