FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693990
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTATTCCGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT43950.6332944278736005No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGACTGGTGATAGTGAGAGTGAAGTCGGTC35360.5095174282050173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24930.35922707819997407No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23830.3433767057162207No Hit
GTTTTATACAGCGTCAATAATAAGTATTACCTAGCTTGGTACCAACAGAA23800.34294442283030013No Hit
GTATAAAACACTCTGTGAGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAAA21270.3064885661176674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19340.27867836712344557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19030.27421144396893327No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17790.256343751350884No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17500.25216501678698544No Hit
CACTATCACCAGTCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTATTCCGACGTTCGG17140.24697762215593883No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15470.2229138748396951No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15460.22276978054438823No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14870.21426821712128416No Hit
GTATTACCTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGCTTC14850.21398002853067047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14740.21239499128229514No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13360.19250997852995No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13100.18876352685197195No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13040.18789896108013085No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12290.1770918889321172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11960.17233677718699117No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11840.170607645643309No Hit
GTTTATTACTGTCAGCAGTATTATAGTATTCCGACGTTCGGCCAAGGGAC11730.16902260839493363No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11670.16815804262309256No Hit
CTCCTAAGCTTCTCATTCACTGGGCATCTACTCGGGAACCCGGGGTCCCT11270.1623942708108186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10720.15446908456894193No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10640.15331633020648713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10100.1455352382599173No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10020.1443824838974625No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9910.14279744664908717No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9460.13631320336027897No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9200.13256675168230092No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9160.1319903745010735No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9150.13184628020576664No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGACTGGTGATAGTGAGAGTGAAGTCGGTCCCAGACCCGCTGCCA9060.13054943154800502No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACCGACTTCACTCTCAC9050.13040533725269815No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8980.12939667718555023No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8900.12824392282309544No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8800.12680297987002695No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8770.1263706969841064No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC8750.12608250839349272No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8720.12565022550757216No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8680.12507384832634477No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAGGTAATACTTATTATTGACGCTGTATAAAACACT8620.12420928255450366No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8610.12406518825919682No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8510.12262424530612834No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8500.12248015101082148No Hit
GTGTTTTATACAGCGTCAATAATAAGTATTACCTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGCTT8370.12060692517183245No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8260.11902188792345711No Hit
AACCAGGACAGCCTCCTAAGCTTCTCATTCACTGGGCATCTACTCGGGAA7970.1148431533595585No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG7940.11441087047363795No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7940.11441087047363795No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7900.11383449329241055No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7770.11196126745342153No Hit
ATTCAGTGGCAGCGGGTCTGGGACCGACTTCACTCTCACTATCACCAGTC7730.11138489027219411No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7710.11109670168158042No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7690.11080851309096672No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7640.11008804161443247No Hit
GTCTGGGACCGACTTCACTCTCACTATCACCAGTCTGCAGCCTGAAGATG7590.10936757013789823No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7190.1036037983256243No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7120.10259513825847635No Hit
AGGTAATACTTATTATTGACGCTGTATAAAACACTCTGTGAGGACTTGCA7090.10216285537255579No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7090.10216285537255579No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7030.10129828960071471No Hit
GCCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTATTCCGA6940.10000144094295307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)