FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693990
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTATTCCGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT41150.5929480251876829No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGACTGGTGATAGTGAGAGTGAAGTCGGTC32480.46801827115664485No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22640.3262294845747057No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21410.3085058862519633No Hit
GTTTTATACAGCGTCAATAATAAGTATTACCTAGCTTGGTACCAACAGAA21000.3025980201443825No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20390.29380826813066474No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18020.25965792014294153No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16980.24467211343102926No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15100.2175823859133417No Hit
CACTATCACCAGTCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTATTCCGACGTTCGG14770.21282727416821567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14410.20763987953716914No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13600.19596824161731435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13540.19510367584547328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12980.18703439530828975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12850.1851611694693007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12830.184872980878687No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12690.1828556607443911No Hit
GTATAAAACACTCTGTGAGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAAA12340.17781236040865142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11850.1707517399386158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11840.170607645643309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11710.16873441980431994No Hit
GTATTACCTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGCTTC11460.16513206242164874No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11220.16167379933428436No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGACTGGTGATAGTGAGAGTGAAGTCGGTCCCAGACCCGCTGCCA10930.15749506477038575No Hit
GTTTATTACTGTCAGCAGTATTATAGTATTCCGACGTTCGGCCAAGGGAC10430.1502903500050433No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9950.14337382383031455No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGGTTCCCGAGT9820.14150059799132553No Hit
CTCCTAAGCTTCTCATTCACTGGGCATCTACTCGGGAACCCGGGGTCCCT9820.14150059799132553No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9750.14049193792417758No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9650.1390509949711091No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9520.13717776913212007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9450.13616910906497212No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9400.13544863758843786No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9380.13516044899782417No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACCGACTTCACTCTCAC8580.12363290537327629No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8480.12219196242020777No Hit
GGTGATAGTGAGAGTGAAGTCGGTCCCAGACCCGCTGCCACTGAATCGGT8440.12161558523898039No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8360.1204628308765256No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8340.1201746422859119No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8190.11801322785630916No Hit
GTGTTTTATACAGCGTCAATAATAAGTATTACCTAGCTTGGTACCAACAGAAACCAGGACAGCCTCCTAAGCTT8120.11700456778916121No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8040.11585181342670643No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAGGTAATACTTATTATTGACGCTGTATAAAACACT8030.1157077191313996No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7880.11354630470179684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC7780.11210536174872836No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7660.11037623020504618No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7580.1092234758425914No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7460.10749434429890921No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7410.10677387282237497No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7400.1066297785270681No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7390.10648568423176127No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7370.10619749564114757No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7350.10590930705053386No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7130.1027392325537832No Hit
TCTTCAGGCTGCAGACTGGTGATAGTGAGAGTGAAGTCGGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC7000.10086600671479418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)