FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59760.3821098911216997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54010.34534396284275437No Hit
CCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTAGCCAAGGCCTCGAA49860.3188085537370808No Hit
GTGTGTATGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC48270.30864197530864196No Hit
ACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT46870.29969027103202917No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41330.2642670983945758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38170.24406182302736407No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36920.23606922992324553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34910.22321714021182293No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34170.2184855250941847No Hit
CATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33150.21196356912122397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31840.20358733154810776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31580.20192487218245106No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30350.1940601605679984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30240.19335681237483598No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA29990.19175829375401227No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC28800.18414934511889142No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA28400.1815917153255735No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG28390.18152777458074054No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27470.17564522605610927No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26550.16976267753147803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26440.16905932933831558No Hit
GTGTATGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC26360.168547803379652No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGGTTGGACATATCCAAATACA26310.16822809965548727No Hit
CAACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT25980.16611805507599997No Hit
GGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTTCTAATCGCGAC25470.1628570770895196No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25400.16240949187568895No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24430.15620723962689295No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC24370.1558235951578953No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA23770.15198715046791839No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23660.15128380227475594No Hit
GGTCTAGCCAAGGCCTCGAAGACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC23440.14987710588843106No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23080.14757523907444492No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT23030.1472555353502802No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTAGCCAAGGCCTCGAAGA22230.14214027576364432No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC21810.1394547644806605No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21800.13939082373582753No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21410.13689713468734255No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21350.13651349021834486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC21240.13581014202518243No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21190.13549043830101767No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCCAGGCGCCTAATTTATAAGGTTT21130.13510679383201998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20800.1329967492525327No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19740.12621903030024018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19670.12577144508640953No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG19660.12570750434157657No Hit
GGCCAAAAGTGTGAGGCCAGTGTGTATGTTGCATGCAGTAATAAACCCCA19160.12251046709992916No Hit
GTATATTACTGTCTGGCCTGGGGGGTGAAAAACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC19040.12174317816193378No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCGTCTCCTGCAGGTCTA18660.11931342985828174No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17900.11445393325097766No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17600.1125357109059892No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT17520.11202418494732562No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17370.1110650737748314No Hit
GTTTATTACTGCATGCAACATACACACTGGCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17130.10953049589884063No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17010.10876320696084525No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16910.10812379951251577No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT16650.10646134014685911No Hit
TTATTACTGCATGCAACATACACACTGGCCTCACACTTTTGGCCAGGGGA16320.1043512955673718No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16180.10345612513971052No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16150.10326430290521169No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16120.10307248067071284No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16040.10256095471204926No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT15990.1022412509878845No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15940.10192154726371976No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15910.10172972502922092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)